Page generated on Fri Oct 15 12:01:49 2010

dme-mir-1013




dme-mir-1013

View dme-mir-1013 on miRBase
miRNALengthPerfect(sense)Perfect(antisense)5' moR5'armLoop3'arm3' moROtherLoop SizeMatureStar
dme-mir-1013110454013411744702817447041


ACCGAAGCCCACCGTGCTGGAGAGGTGAGTTTCGTACACTTAATTAATAGGATCGGCCGTTAATAAAAGTATGCCGAACTCGCAGGCGACGGATGCGCGATCACCAAAGA
........................((((((((.(((.((((.((((((.((.....))))))))..)))).))).))))))))...........................-18.70
...((.((.(((((((((......((((((((.(((.((((.((((((.((.....))))))))..)))).))).)))))))).))).)))).)).))..))........-31.50
Sense strand
                                                                                                              SizeBlastTotalV024 kc167 cellV018 Kc167 cellSRR031696_2152 SRR029633 total small RNAs from hen1 homozygous flies V081 embryo 2-6hrS29 male head #1V097 CS ovarySRR014278 Ovary_rep1 w1118(fem)_x_Har(male)_F1 V082 embryo 14-24hrV098 dcr-2[L811fsX] ovaryV067 S2-NPV076 ML-DmBG3-C2SRR031703 Total small RNAs from r2d2 homozygous flies V065 dcr-2[L811fsX], ovary, AGO1IPV033 CMEW1 Cl.8+ cellSRR031701 Total small RNAs from r2d2 heterozygous flies SRR031697 Total small RNAs from dcr-2 heterozygous flies SRR031692 Total small RNAs from Oregon R V009 CMEL1GSM379065 Zuc Heterozygote V090 CS  male total RNA  SRR014274 Ovary_rep1 LK(fem)_x_Har(male)_F1 GSM280085 WT testes (18-24nt) V083 male, one dayGSM379057 Krimp Mutant SRR031699 Total small RNAs from dcr-2 homozygous flies SRR014276 Ovary_rep1 NA(fem)_x_Har(male)_F1 V063 CS,ovary,AGO1IPV075 ML-DmBG1-C1S13 6-10h #2 (11)SRR014280 Ovary_rep1 w1118_P V021 ML-DmD21 cellV064 ago2[414], ovary, AGO1IPGSM280088 S2cell (AGO1IP) SRR014282 Ovary_rep1 wK_P SRR014281 Ovary_rep1 wK(fem)_x_w1118(male)_F1 S12 6-10h #1 (10)V088 r2d2[1] ovary total RNA  S22 female head #1SRR001338 IR non-beta-eliminated SRR029028 untreated (mock) GSM379066 Zuc Mutant V084 female, one dayV028 CMEW1 Cl.8+ cellSRR014279 Ovary_rep1 w1118(fem)_x_wK(male)_F1 V026 1182-4H cellSRR001345 ago2 non-beta-eliminated GSM379064 Vasa Mutant V001 IR-S32 s2+48 #2V010 MLDmD20c5GSM379062 Squ Mutant GSM379061 Squ Heterozygote V066 r2d2[1], ovary, AGO1IPSRR014277 Ovary_rep1 NA_P S33 KC-48 #2SRR014270 Embryo_0-2hrs rep1_NA_0_2hr_Emb GSM379051 Armi Mutant SRR014269 Embryo_0-2hrs rep1_LK_0_2hr_Emb SRR014275 Ovary_rep1 LK_P SRR031704 2-O-methylated small RNAs from r2d2 homozygous flies S31 s2+48 #1SRR014272 Embryo_0-2hrs rep1_wK_0_2hr_Emb GSM379063 Vasa Heterozygote V089 ago2[414] ovary total RNA  V019 GM2 cellSRR023197 RNA Library from S2 control cells GSM379060 SpnE Heterozygote S24 male body #2GSM379050 Armi Heterozygote GSM379053 Aub Mutant V008 S2-DRSCV002 IR+SRR014273 Ovary_rep1_Har_P GSM379058 S10 2-6h #1 (8)GSM379052 Aub Heterozygote S1 male headSRR029030 dcr-2 knockdown S28 0-2d pupaeS35 KC+48 #2GSM379056 Krimp Heterozygote S23 female body #2SRR031700 2-O-methylated small RNAs from dcr-2 homozygous flies SRR010959 Ago3 IP in heterozygotes S2 female headSRR001347 ago2 untreated SRR014268 Embryo_0-2hrs rep1_HAR_0_2hr_Emb GSM343287 Drosophila Toll 10b mutant embryos OSS6 Drosophila OSS cell small RNA libraries, rep2 SRR001341 WT_males non-beta-eliminated SRR001343 dcr-2 non-beta-eliminated SRR032095 AGO1 IP dcr2 knockdown SRR001339 WT_females non-beta-eliminated V011 Sg4GSM379059 GSM379067 SpnE Mutant S11 2-6h #2 (9)S9 0-1h #3 (7)S21 disk #2S30 s2-48 #2SRR001664 homozygous dcr-2_untreated GSM280083 dcr-2-/- ovaries (18-29nt) SRR001349 heterozygous dcr-2_untreated GSM379054 Flam Heterozygote SRR001344 dcr-2 beta-eliminated SRR014271 Embryo_0-2hrs rep1_w1118_0_2hr_Emb OSS8 Drosophila OSS cell small RNA libraries, rep4 GSM180328 adult heads (female heads, male heads) S34 KC+48 #1OSS2 Drosophila OSS cell small RNA libraries, rep1 S8 S2-48,+48, KC-48, +48 mixOSS7 Drosophila OSS cell small RNA libraries, rep3 V025 S2R+ cellS19 2-4day pupae #1SRR032094 ago2 knockdown S16 KC -48 #1SRR010957 Aub IP in Ago3 trans-heterozygotes SRR029033 lacZ knockdown SRR023400 total RNA extracted from P19 cells SRR023407 RNA bound by NLS-P19 protein SRR023399 RNA bound by P19 protein S27 0-1d Pupae (w)SRR010956 Piwi IP in Ago3 heterozygotes V020 S2R+ cellGSM467729 WT S3 male bodyGSM180331 early embryo (2-6) GSM467730 r2d2 SRR010953 Aub heterozygotes, oxidized GSM180333 late embryo (12-24) GSM180332 mid embryo (6-10) S4 female bodySRR029031 loqs-ORF knockdown S7 12-24hr embryoSRR031693_4 SRR001337 WT_females beta-eliminated GSM180337 tissue culture cells (S2 only) GSM280084 loqs-/- ovaries (18-29nt) GSM246084 D. melanogaster adult male heads 454 S5 imaginal discSRR010955 Aub IP in Ago3 heterozygotes GSM239052 S2 cells, non beta-eliminated SRR032093 ago1 knockdown S14 0-1hr #1 (A)GSM180335 imaginal discs GSM180336 pupae: 0-1 day, 0-2 day, 2-4 day SRR010954 Aub trans-heterozygotes, oxidized SRR031702 2-O-methylated small RNAs from r2d2 heterozygous flies GSM180330 very early embryo (0-1) S15 S2 -48 #1S20 2-4day pupae#2SRR010958 Piwi IP in Ago3 trans-heterozygotes GSM280082 WT ovaries (18-29nt) S17 3rd inster #1GSM280087 S2cell (AGO2IP) GSM280086 WT ovaries (AGO2IP) S26 1st instar #2GSM239051 S2 cells, beta-eliminated GSM154621 piRNAs associated with Ago3 from Drosophila melanogaster ovaries SRR010952 Ago3 trans-heterozygotes, oxidized SRR032092 mock oxidized GSM424740 S2 pKF63 stably transfected SRR010960 wt, oxidized SRR023402 total RNA extracted from NLS-P19 cells SRR001340 IR beta-eliminated S18 3rd inster #2GSM467731 loq GSM154622 piRNAs associated with Aubergine from Drosophila melanogaster ovaries GSM154620 piRNAs associated with Piwi from Drosophila melanogaster ovaries SRR001342 WT_males beta-eliminated GSM154618 23-29 nucleotide RNAs from Drosophila melanogaster ovaries GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006) GSM239050 fly heads, beta-eliminated GSM379055 Flam Mutant SRR031698 2-O-methylated small RNAs from dcr-2 heterozygous flies SRR029029 dcr-1 knockdown GSM180334 larvae: 1st instar and 3rd instars S25 1st instar #1SRR029032 r2d2 knockdown GSM424741 S2 pKF63 transiently transfected SRR032096 AGO2 IP dcr2 knockdownGSM266765 Siomi Lab fruit fly S2 AGO2 Cloned Sequences v1.0 SRR001348 ago2 oxidized GSM239041 fly heads, non beta-eliminated GSM424739 S2 parental GSM180329 adult bodies (female bodies, male bodies) SRR001346 ago2 beta-eliminated SRR010951 Ago3 heterozygotes, oxidized
......GCCCACCGTGCTGGAGAG......................................................................................1813000000000000000000000000000000000000000000000000000000000000020000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
........................GTGAGTTTCGTACACTTAATTA................................................................22111200120000000001011000000000000000000200000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000
........................GTGAGTTTCGTACACTTAATT.................................................................21122020520000000001000000100000000000000001000100000100000000000400000000000100000000000000000000000000000100000000000000000000000000000010100000000000000000000000000000000000000000000000000000
........................GTGAGTTTCGTACACTTAATTAATAGG...........................................................2712000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000
........................GTGAGTTTCGTACACTTA....................................................................1813000000000000000000001000000000010000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
........................GTGAGTTTCGTACACTTAAT..................................................................2015010000000011000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000
........................GTGAGTTTCGTACACTTAATTAATAGGAT.........................................................2912000000000000000001000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.........................................AATTAATAGGATCGGCCGTTAATA.............................................2411000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...........................................TTAATAGGATCGGCCGTTAAT..............................................2111000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
............................................TAATAGGATCGGCCGTTAAT..............................................2016002010000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000200000000000000000000000000000000000000000000000000000000000000000000000000000000
............................................TAATAGGATCGGCCGTTAATAAAAGT........................................2611000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................................AATAGGATCGGCCGTTAA...............................................1813000010000010000000000000000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................................AATAGGATCGGCCGTTAAT..............................................1916000000000000100011000000000000000200000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...............................................TAGGATCGGCCGTTAATAAA...........................................2011000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................................................AATAAAAGTATGCCGAACTCG............................2111000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................................................AATAAAAGTATGCCGAACTCGC...........................2216000000000000000000000000000003000000001000000000000000000000000000000000000000000000000000000000000010000000000100000000000000000000000000000000000000000000000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACTCGCAG.........................231128134197547107782759502681239322625233222108303156181614112021159314621749083910487121212289074471000552864622557933460222041014220222302101211101011022000303320100010011100010100020101000011110000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACTCGCA..........................221288963317938510903588874834255380411116102751402091540301413000111000100012020100011000102000012120100000000010100101010000000000110110000000000000000000000000000000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACTCGCAGG........................2411000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACTCG............................2011048634732377225830175754351815914104821226281723511121116612201311187275102175531513943965750126858121332010224717320204360025014004340310200130211202000001021110000010001000001000001100000000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACT..............................1816002000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000001200000000000000000000000000000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACTC.............................19125003200000020200003000010001000000000002100100001000000000000000000001000000000000002000000000000000000100000000000000000000000000000000000000000000110000000000000000000000000000000000000000
..............................................................ATAAAAGTATGCCGAACTCGC...........................21113618313840552938604353362324121126182117181716101410151118111914916851522310148581210127694317310510126856439374454376574143031232352202521541371222014234112101242040002101101001002111012200010000000000000000000000000000000000000000000
...............................................................TAAAAGTATGCCGAACTCGCAG.........................22130253261324142168333473020432432341404404663000207111403231112001220003113412201101010010100010301000011100003010030000001000101121000000010100000100000000000000000000000000000000000000000000000000
...............................................................TAAAAGTATGCCGAACTCGCA..........................2116911135004011212110100232000020040011010002101100010001000000000000000000001000000000100001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...............................................................TAAAAGTATGCCGAACTC.............................1811000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000000000
...............................................................TAAAAGTATGCCGAACTCGC...........................201341042000000020000200000101000100000200000001000110000000000000010000000000000000000000000010000000000100001000000010000000000000000000000000010000000000000000000000000000000000000000000000000
...............................................................TAAAAGTATGCCGAACTCG............................1914010000000000000000000000000000000000000000000000010000000000000000000000000000000000000000001000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
................................................................AAAAGTATGCCGAACTCGCAG.........................21133343000011012000001011000000000000000000101003010000000001000000000000000000000001000500100000000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000
................................................................AAAAGTATGCCGAACTCGCAGG........................2211000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000
.................................................................AAAGTATGCCGAACTCGCAG.........................2013000000000000000010010000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
..................................................................AAGTATGCCGAACTCGCAG.........................1912000002000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...................................................................AGTATGCCGAACTCGCAG.........................1814000000000000000001000000000000000000000000000000000000000000000000000000000000000030000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
....................................................................................GGCGACGGATGCGCGATCACC.....2112000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...........................................................................................GATGCGCGATCACCAAAGA1911000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
Antisense strand
                                                                                                              SizeBlastTotalV024 kc167 cellV018 Kc167 cellSRR031696_2152 SRR029633 total small RNAs from hen1 homozygous flies V081 embryo 2-6hrS29 male head #1V097 CS ovarySRR014278 Ovary_rep1 w1118(fem)_x_Har(male)_F1 V082 embryo 14-24hrV098 dcr-2[L811fsX] ovaryV067 S2-NPV076 ML-DmBG3-C2SRR031703 Total small RNAs from r2d2 homozygous flies V065 dcr-2[L811fsX], ovary, AGO1IPV033 CMEW1 Cl.8+ cellSRR031701 Total small RNAs from r2d2 heterozygous flies SRR031697 Total small RNAs from dcr-2 heterozygous flies SRR031692 Total small RNAs from Oregon R V009 CMEL1GSM379065 Zuc Heterozygote V090 CS  male total RNA  SRR014274 Ovary_rep1 LK(fem)_x_Har(male)_F1 GSM280085 WT testes (18-24nt) V083 male, one dayGSM379057 Krimp Mutant SRR031699 Total small RNAs from dcr-2 homozygous flies SRR014276 Ovary_rep1 NA(fem)_x_Har(male)_F1 V063 CS,ovary,AGO1IPV075 ML-DmBG1-C1S13 6-10h #2 (11)SRR014280 Ovary_rep1 w1118_P V021 ML-DmD21 cellV064 ago2[414], ovary, AGO1IPGSM280088 S2cell (AGO1IP) SRR014282 Ovary_rep1 wK_P SRR014281 Ovary_rep1 wK(fem)_x_w1118(male)_F1 S12 6-10h #1 (10)V088 r2d2[1] ovary total RNA  S22 female head #1SRR001338 IR non-beta-eliminated SRR029028 untreated (mock) GSM379066 Zuc Mutant V084 female, one dayV028 CMEW1 Cl.8+ cellSRR014279 Ovary_rep1 w1118(fem)_x_wK(male)_F1 V026 1182-4H cellSRR001345 ago2 non-beta-eliminated GSM379064 Vasa Mutant V001 IR-S32 s2+48 #2V010 MLDmD20c5GSM379062 Squ Mutant GSM379061 Squ Heterozygote V066 r2d2[1], ovary, AGO1IPSRR014277 Ovary_rep1 NA_P S33 KC-48 #2SRR014270 Embryo_0-2hrs rep1_NA_0_2hr_Emb GSM379051 Armi Mutant SRR014269 Embryo_0-2hrs rep1_LK_0_2hr_Emb SRR014275 Ovary_rep1 LK_P SRR031704 2-O-methylated small RNAs from r2d2 homozygous flies S31 s2+48 #1SRR014272 Embryo_0-2hrs rep1_wK_0_2hr_Emb GSM379063 Vasa Heterozygote V089 ago2[414] ovary total RNA  V019 GM2 cellSRR023197 RNA Library from S2 control cells GSM379060 SpnE Heterozygote S24 male body #2GSM379050 Armi Heterozygote GSM379053 Aub Mutant V008 S2-DRSCV002 IR+SRR014273 Ovary_rep1_Har_P GSM379058 S10 2-6h #1 (8)GSM379052 Aub Heterozygote S1 male headSRR029030 dcr-2 knockdown S28 0-2d pupaeS35 KC+48 #2GSM379056 Krimp Heterozygote S23 female body #2SRR031700 2-O-methylated small RNAs from dcr-2 homozygous flies SRR010959 Ago3 IP in heterozygotes S2 female headSRR001347 ago2 untreated SRR014268 Embryo_0-2hrs rep1_HAR_0_2hr_Emb GSM343287 Drosophila Toll 10b mutant embryos OSS6 Drosophila OSS cell small RNA libraries, rep2 SRR001341 WT_males non-beta-eliminated SRR001343 dcr-2 non-beta-eliminated SRR032095 AGO1 IP dcr2 knockdown SRR001339 WT_females non-beta-eliminated V011 Sg4GSM379059 GSM379067 SpnE Mutant S11 2-6h #2 (9)S9 0-1h #3 (7)S21 disk #2S30 s2-48 #2SRR001664 homozygous dcr-2_untreated GSM280083 dcr-2-/- ovaries (18-29nt) SRR001349 heterozygous dcr-2_untreated GSM379054 Flam Heterozygote SRR001344 dcr-2 beta-eliminated SRR014271 Embryo_0-2hrs rep1_w1118_0_2hr_Emb OSS8 Drosophila OSS cell small RNA libraries, rep4 GSM180328 adult heads (female heads, male heads) S34 KC+48 #1OSS2 Drosophila OSS cell small RNA libraries, rep1 S8 S2-48,+48, KC-48, +48 mixOSS7 Drosophila OSS cell small RNA libraries, rep3 V025 S2R+ cellS19 2-4day pupae #1SRR032094 ago2 knockdown S16 KC -48 #1SRR010957 Aub IP in Ago3 trans-heterozygotes SRR029033 lacZ knockdown SRR023400 total RNA extracted from P19 cells SRR023407 RNA bound by NLS-P19 protein SRR023399 RNA bound by P19 protein S27 0-1d Pupae (w)SRR010956 Piwi IP in Ago3 heterozygotes V020 S2R+ cellGSM467729 WT S3 male bodyGSM180331 early embryo (2-6) GSM467730 r2d2 SRR010953 Aub heterozygotes, oxidized GSM180333 late embryo (12-24) GSM180332 mid embryo (6-10) S4 female bodySRR029031 loqs-ORF knockdown S7 12-24hr embryoSRR031693_4 SRR001337 WT_females beta-eliminated GSM180337 tissue culture cells (S2 only) GSM280084 loqs-/- ovaries (18-29nt) GSM246084 D. melanogaster adult male heads 454 S5 imaginal discSRR010955 Aub IP in Ago3 heterozygotes GSM239052 S2 cells, non beta-eliminated SRR032093 ago1 knockdown S14 0-1hr #1 (A)GSM180335 imaginal discs GSM180336 pupae: 0-1 day, 0-2 day, 2-4 day SRR010954 Aub trans-heterozygotes, oxidized SRR031702 2-O-methylated small RNAs from r2d2 heterozygous flies GSM180330 very early embryo (0-1) S15 S2 -48 #1S20 2-4day pupae#2SRR010958 Piwi IP in Ago3 trans-heterozygotes GSM280082 WT ovaries (18-29nt) S17 3rd inster #1GSM280087 S2cell (AGO2IP) GSM280086 WT ovaries (AGO2IP) S26 1st instar #2GSM239051 S2 cells, beta-eliminated GSM154621 piRNAs associated with Ago3 from Drosophila melanogaster ovaries SRR010952 Ago3 trans-heterozygotes, oxidized SRR032092 mock oxidized GSM424740 S2 pKF63 stably transfected SRR010960 wt, oxidized SRR023402 total RNA extracted from NLS-P19 cells SRR001340 IR beta-eliminated S18 3rd inster #2GSM467731 loq GSM154622 piRNAs associated with Aubergine from Drosophila melanogaster ovaries GSM154620 piRNAs associated with Piwi from Drosophila melanogaster ovaries SRR001342 WT_males beta-eliminated GSM154618 23-29 nucleotide RNAs from Drosophila melanogaster ovaries GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006) GSM239050 fly heads, beta-eliminated GSM379055 Flam Mutant SRR031698 2-O-methylated small RNAs from dcr-2 heterozygous flies SRR029029 dcr-1 knockdown GSM180334 larvae: 1st instar and 3rd instars S25 1st instar #1SRR029032 r2d2 knockdown GSM424741 S2 pKF63 transiently transfected SRR032096 AGO2 IP dcr2 knockdownGSM266765 Siomi Lab fruit fly S2 AGO2 Cloned Sequences v1.0 SRR001348 ago2 oxidized GSM239041 fly heads, non beta-eliminated GSM424739 S2 parental GSM180329 adult bodies (female bodies, male bodies) SRR001346 ago2 beta-eliminated SRR010951 Ago3 heterozygotes, oxidized
...........................................................................................GATGCGCGATCACCAAAGA1911000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000