Page generated on Fri Oct 15 12:01:13 2010

dme-mir-13b-1



dme-mir-13b-1

View dme-mir-13b-1 on miRBase

miRNA	Length	Perfect(sense)	Perfect(antisense)	5' moR	5'arm	Loop	3'arm	3' moR	Other	Loop Size	Mature	Star
dme-mir-13b-1	117	5169090	3	2	1290		5167794	1	6	12	5167794	1290

TGACTGAACCACGTCTTTCTTATACCATGTCGTTAAAATGTTTGTGAACTTATGTATTCACAATCATATCACAGCCATTTTGACGAGTTTGGGTAAAGAAATCTCAACATTGAACGG
........................(((..(((((((((((.((((((...((((..........)))))))))).)))))))))))...))).........................-21.20
.(((........))).(((((.((((...(((((((((((.((((((...((((..........)))))))))).))))))))))).....))))))))).................-28.00
Sense strand

	Size	Blast	Total	V065 dcr-2[L811fsX], ovary, AGO1IP	V098 dcr-2[L811fsX] ovary	V066 r2d2[1], ovary, AGO1IP	V063 CS,ovary,AGO1IP	V088 r2d2[1] ovary total RNA	V064 ago2[414], ovary, AGO1IP	V097 CS ovary	V076 ML-DmBG3-C2	V089 ago2[414] ovary total RNA	SRR032095 AGO1 IP dcr2 knockdown	V081 embryo 2-6hr	V082 embryo 14-24hr	V067 S2-NP	SRR031696_2152	SRR014281 Ovary_rep1 wK(fem)_x_w1118(male)_F1	SRR014274 Ovary_rep1 LK(fem)_x_Har(male)_F1	V024 kc167 cell	V026 1182-4H cell	V019 GM2 cell	GSM379057 Krimp Mutant	SRR014280 Ovary_rep1 w1118_P	GSM280088 S2cell (AGO1IP)	GSM379065 Zuc Heterozygote	GSM379064 Vasa Mutant	GSM424740 S2 pKF63 stably transfected	S33 KC-48 #2	SRR014276 Ovary_rep1 NA(fem)_x_Har(male)_F1	S32 s2+48 #2	GSM379063 Vasa Heterozygote	SRR014275 Ovary_rep1 LK_P	V033 CMEW1 Cl.8+ cell	SRR014271 Embryo_0-2hrs rep1_w1118_0_2hr_Emb	V011 Sg4	SRR029028 untreated (mock)	V021 ML-DmD21 cell	SRR001345 ago2 non-beta-eliminated	V018 Kc167 cell	V008 S2-DRSC	SRR031703 Total small RNAs from r2d2 homozygous flies	SRR014279 Ovary_rep1 w1118(fem)_x_wK(male)_F1	V009 CMEL1	SRR014278 Ovary_rep1 w1118(fem)_x_Har(male)_F1	SRR014270 Embryo_0-2hrs rep1_NA_0_2hr_Emb	SRR014277 Ovary_rep1 NA_P	GSM379056 Krimp Heterozygote	SRR029633 total small RNAs from hen1 homozygous flies	SRR014282 Ovary_rep1 wK_P	GSM379066 Zuc Mutant	GSM379060 SpnE Heterozygote	V025 S2R+ cell	V090 CS  male total RNA	SRR014269 Embryo_0-2hrs rep1_LK_0_2hr_Emb	GSM379052 Aub Heterozygote	SRR031701 Total small RNAs from r2d2 heterozygous flies	SRR014272 Embryo_0-2hrs rep1_wK_0_2hr_Emb	V028 CMEW1 Cl.8+ cell	S31 s2+48 #1	GSM379062 Squ Mutant	SRR014273 Ovary_rep1_Har_P	SRR031692 Total small RNAs from Oregon R	SRR031697 Total small RNAs from dcr-2 heterozygous flies	GSM379051 Armi Mutant	SRR029030 dcr-2 knockdown	GSM379061 Squ Heterozygote	SRR031699 Total small RNAs from dcr-2 homozygous flies	V083 male, one day	S29 male head #1	V002 IR+	SRR032094 ago2 knockdown	GSM379058	SRR001349 heterozygous dcr-2_untreated	V020 S2R+ cell	OSS6 Drosophila OSS cell small RNA libraries, rep2	V084 female, one day	SRR029031 loqs-ORF knockdown	V001 IR-	S30 s2-48 #2	GSM379067 SpnE Mutant	OSS2 Drosophila OSS cell small RNA libraries, rep1	GSM379054 Flam Heterozygote	V010 MLDmD20c5	SRR029032 r2d2 knockdown	GSM379050 Armi Heterozygote	OSS7 Drosophila OSS cell small RNA libraries, rep3	OSS8 Drosophila OSS cell small RNA libraries, rep4	V075 ML-DmBG1-C1	SRR032093 ago1 knockdown	SRR001338 IR non-beta-eliminated	GSM379053 Aub Mutant	S35 KC+48 #2	S15 S2 -48 #1	GSM280084 loqs-/- ovaries (18-29nt)	SRR001664 homozygous dcr-2_untreated	S16 KC -48 #1	GSM280083 dcr-2-/- ovaries (18-29nt)	SRR029033 lacZ knockdown	GSM343287 Drosophila Toll 10b mutant embryos	SRR001347 ago2 untreated	S34 KC+48 #1	SRR014268 Embryo_0-2hrs rep1_HAR_0_2hr_Emb	SRR029029 dcr-1 knockdown	GSM379059	SRR001344 dcr-2 beta-eliminated	S24 male body #2	S12 6-10h #1 (10)	S28 0-2d pupae	S9 0-1h #3 (7)	S10 2-6h #1 (8)	S13 6-10h #2 (11)	S27 0-1d Pupae (w)	GSM280082 WT ovaries (18-29nt)	S14 0-1hr #1 (A)	GSM280085 WT testes (18-24nt)	GSM379055 Flam Mutant	S22 female head #1	S23 female body #2	S2 female head	S8 S2-48,+48, KC-48, +48 mix	S19 2-4day pupae #1	SRR001341 WT_males non-beta-eliminated	SRR001339 WT_females non-beta-eliminated	SRR001343 dcr-2 non-beta-eliminated	GSM424739 S2 parental	SRR010954 Aub trans-heterozygotes, oxidized	S21 disk #2	S5 imaginal disc	SRR031704 2-O-methylated small RNAs from r2d2 homozygous flies	S7 12-24hr embryo	S11 2-6h #2 (9)	S1 male head	SRR010953 Aub heterozygotes, oxidized	SRR001348 ago2 oxidized	SRR010959 Ago3 IP in heterozygotes	S4 female body	GSM239052 S2 cells, non beta-eliminated	S18 3rd inster #2	GSM239041 fly heads, non beta-eliminated	SRR032092 mock oxidized	SRR031698 2-O-methylated small RNAs from dcr-2 heterozygous flies	GSM467729 WT	SRR031700 2-O-methylated small RNAs from dcr-2 homozygous flies	GSM424741 S2 pKF63 transiently transfected	S17 3rd inster #1	GSM180337 tissue culture cells (S2 only)	SRR031702 2-O-methylated small RNAs from r2d2 heterozygous flies	SRR001346 ago2 beta-eliminated	GSM467730 r2d2	GSM180328 adult heads (female heads, male heads)	S3 male body	SRR032096 AGO2 IP dcr2 knockdown	S20 2-4day pupae#2	SRR001337 WT_females beta-eliminated	GSM280087 S2cell (AGO2IP)	S25 1st instar #1	GSM180333 late embryo (12-24)	SRR010956 Piwi IP in Ago3 heterozygotes	SRR031693_4	GSM180332 mid embryo (6-10)	GSM180335 imaginal discs	SRR010955 Aub IP in Ago3 heterozygotes	GSM180330 very early embryo (0-1)	GSM180331 early embryo (2-6)	S26 1st instar #2	GSM467731 loq	GSM180329 adult bodies (female bodies, male bodies)	SRR010958 Piwi IP in Ago3 trans-heterozygotes	GSM180334 larvae: 1st instar and 3rd instars	GSM246084 D. melanogaster adult male heads 454	GSM180336 pupae: 0-1 day, 0-2 day, 2-4 day	SRR001340 IR beta-eliminated	SRR010960 wt, oxidized	SRR023400 total RNA extracted from P19 cells	SRR023399 RNA bound by P19 protein	SRR023197 RNA Library from S2 control cells	GSM154618 23-29 nucleotide RNAs from Drosophila melanogaster ovaries	SRR001342 WT_males beta-eliminated	SRR010951 Ago3 heterozygotes, oxidized	SRR010952 Ago3 trans-heterozygotes, oxidized	GSM239050 fly heads, beta-eliminated	GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006)	GSM154621 piRNAs associated with Ago3 from Drosophila melanogaster ovaries	SRR010957 Aub IP in Ago3 trans-heterozygotes	SRR023407 RNA bound by NLS-P19 protein	GSM280086 WT ovaries (AGO2IP)	GSM239051 S2 cells, beta-eliminated	SRR023402 total RNA extracted from NLS-P19 cells	GSM266765 Siomi Lab fruit fly S2 AGO2 Cloned Sequences v1.0	GSM154622 piRNAs associated with Aubergine from Drosophila melanogaster ovaries	GSM154620 piRNAs associated with Piwi from Drosophila melanogaster ovaries
...........................TGTCGTTAAAATGTTTGTGAA.....................................................................	21	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................TGTCGTTAAAATGTTTGTGA......................................................................	20	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAACTT..................................................................	22	1	155	0	0	0	0	0	0	0	0	0	0	5	2	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0	0	3	0	0	14	0	0	0	0	0	0	19	0	0	0	0	0	0	0	4	0	0	0	0	0	18	17	0	0	0	18	0	1	0	0	0	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	7	0	0	0	2	6	0	0	0	0	2	0	3	0	0	0	1	0	0	0	0	0	1	0	1	0	1	0	0	3	0	0	0	0	1	0	0	0	0	3	0	0	0	0	0	0	1	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAAC....................................................................	20	1	19	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	1	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAACTTATGT..............................................................	26	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGA......................................................................	18	1	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	1	0	0	0	0	0	0	0	2	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAACT...................................................................	21	1	117	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	12	0	0	0	0	0	0	20	0	0	0	0	5	0	0	10	0	1	0	0	0	6	16	0	0	0	15	1	6	0	0	0	3	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	8	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAACTTA.................................................................	23	1	18	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	2	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAA.....................................................................	19	1	8	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAACTTAT................................................................	24	1	57	0	0	0	0	0	0	0	0	0	0	2	2	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	5	0	0	0	0	0	0	3	0	0	0	0	0	0	0	6	0	0	0	0	0	5	2	0	0	0	3	0	7	0	0	0	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	2	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1	0	0	6	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGTTAAAATGTTTGTGAACTTATG...............................................................	25	1	870	0	0	0	0	0	0	0	2	0	0	324	74	0	3	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	32	0	0	0	4	0	0	37	0	0	0	27	0	0	16	0	0	0	0	1	2	0	24	0	0	0	0	0	8	30	0	0	0	26	3	7	18	0	0	6	0	0	3	0	32	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	2	0	0	0	0	6	0	1	0	0	1	0	41	3	2	38	15	1	0	2	0	0	1	0	1	0	1	0	0	3	0	0	0	0	17	4	21	1	0	1	0	0	0	0	0	0	2	0	3	0	0	0	7	0	0	0	0	0	0	0	0	0	0	0	10	1	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................CGTTAAAATGTTTGTGAACTTATG...............................................................	24	1	11	0	0	0	0	0	0	0	0	0	0	6	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................CGTTAAAATGTTTGTGAACTTATGT..............................................................	25	1	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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.................................................................ATATCACAGCCATTTTGAC.................................	19	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................ATATCACAGCCATTTTGACGA...............................	21	2	48	0	0	0	0	0	0	0	1	0	0	0	1	0	3	0	1	1	0	0	0	0	0	0	0	0	0	0	3	1	0	0	0	1	0	0	1	1	2	4	0	0	2	0	0	0	3	0	0	0	0	0	0	0	2	0	0	0	0	0	1	0	0	0	0	1	0	5	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	2	0	1	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................TATCACAGCCATTTTGACGAGTTT...........................	24	2	91449	5151	7822	4269	3837	3775	5986	3133	2535	1813	4598	1125	539	1943	1288	955	1111	1328	1515	1251	682	713	2627	623	596	1	392	1056	490	654	1036	517	554	636	1081	431	385	669	1406	709	438	484	1146	660	689	366	424	396	345	303	715	927	678	233	516	441	244	283	302	520	295	276	180	507	343	256	526	43	122	669	138	210	474	304	414	1346	132	284	144	314	137	169	601	168	259	265	248	191	116	69	76	163	117	129	83	124	279	8	123	52	55	207	84	82	74	31	28	54	59	16	21	46	32	4	50	78	61	20	34	33	28	28	30	0	0	15	10	25	0	14	3	0	15	9	15	25	5	13	36	9	151	10	0	1	10	14	6	124	1	8	7	7	6	9	1	1	0	2	2	4	0	1	0	0	28	0	0	1	0	3	2	0	2	1	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0
..................................................................TATCACAGCCATTTTGACGAG..............................	21	2	77097	1235	1021	1005	1256	1204	809	692	1623	326	804	411	217	337	2458	271	403	597	608	1393	553	365	363	426	709	4612	1736	274	1408	271	393	284	499	854	324	700	626	1185	2190	815	320	1194	509	516	369	367	871	196	245	257	475	199	499	423	655	437	157	1034	201	277	528	1219	262	442	51	1277	74	2622	138	796	261	439	665	547	37	178	113	617	148	569	270	767	628	212	456	445	152	962	211	213	510	311	292	248	361	100	243	5127	268	551	777	385	210	162	578	119	151	494	224	250	99	48	492	134	110	1240	273	512	162	71	91	91	68	564	5	93	67	109	71	124	306	13	39	24	55	59	23	89	3	94	55	66	65	28	13	61	18	47	96	42	31	15	23	14	28	27	17	28	10	7	9	17	25	26	27	16	4	2	10	6	1	2	3	0	3	0	1	5	1	0	1	0	0	0	0	0	0	0	0	0
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..................................................................TATCACAGCCATTTTGACGAGTTTG..........................	25	2	269	23	13	14	29	11	27	5	3	7	2	1	1	1	1	3	2	0	2	0	2	1	0	1	3	0	3	0	1	1	5	1	7	1	0	3	0	2	1	1	2	1	2	5	2	1	0	4	0	3	1	0	4	3	1	1	1	0	1	0	0	0	3	0	1	0	0	0	0	0	2	1	0	4	0	0	0	0	0	3	0	0	0	1	0	1	1	0	0	1	0	0	1	1	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	4	0	0	0	0	0	0	14	0	0	0	0	0	0	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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.....................................................................CACAGCCATTTTGACGAG..............................	18	2	22	1	0	1	0	1	0	1	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	2	0	0	0	0	0	0	0	1	0	1	0	1	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................CACAGCCATTTTGACGAGTT............................	20	2	1415	73	29	65	554	31	69	13	10	5	17	7	17	9	11	9	29	4	7	10	1	7	24	2	7	0	9	22	6	0	12	8	0	1	13	2	1	7	2	2	4	0	10	2	10	3	2	2	6	3	1	0	7	4	0	0	3	7	0	11	1	6	1	28	2	4	2	0	5	3	0	1	4	22	4	2	4	5	2	19	1	0	11	1	12	22	0	11	1	0	0	2	0	0	0	7	33	0	1	0	0	5	0	0	0	0	0	0	0	0	0	4	0	2	2	0	1	0	3	5	0	0	0	0	0	1	0	0	0	0	0	0	0	0	2	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................ACAGCCATTTTGACGAGTTTG..........................	21	2	22	3	1	1	2	0	8	0	1	1	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................ACAGCCATTTTGACGAGT.............................	18	2	18102	876	76	661	785	55	969	40	158	23	462	94	188	263	118	301	333	208	198	256	153	316	386	149	175	0	0	293	8	218	266	114	406	108	96	225	932	185	113	38	341	94	294	467	256	81	365	165	218	229	112	9	317	230	45	266	102	0	141	189	178	265	146	29	155	265	9	0	117	21	153	6	101	187	7	107	100	2	109	82	142	53	60	142	177	73	1	19	118	77	0	0	117	8	0	70	22	0	7	0	166	41	88	184	0	0	0	0	0	0	0	43	0	4	63	0	0	0	0	0	50	50	50	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	20	8	0	3	0	0	0	0	13	1	0	0	1	0	7	8	0	0	0	2	0	0	0	0	0	0	0	0	2	0	0	0	1	0	1	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
......................................................................ACAGCCATTTTGACGAGTTT...........................	20	2	1129	54	9	43	50	1	88	2	15	4	31	3	4	8	25	29	26	14	12	13	9	17	61	8	11	0	0	23	0	15	29	8	20	3	5	2	47	6	12	3	14	1	31	19	14	5	29	11	13	4	3	3	23	6	7	11	2	0	7	8	10	14	2	10	5	12	1	0	2	2	1	1	3	13	0	25	2	0	2	15	1	1	3	4	17	8	0	1	8	2	0	0	0	2	0	5	5	0	0	0	3	6	0	8	0	0	0	0	0	0	0	3	0	0	0	0	0	1	0	0	1	1	5	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................ACAGCCATTTTGACGAGTT............................	19	2	40933	3319	394	3098	3523	308	3322	173	379	96	178	160	237	607	278	776	1235	375	345	350	625	674	785	450	613	0	1	915	6	810	904	432	365	157	319	187	285	181	138	41	605	164	475	404	663	269	429	448	640	591	200	30	414	383	35	226	238	5	420	378	230	284	336	98	582	295	17	0	165	92	347	21	112	401	29	115	144	0	285	232	209	56	141	246	362	232	7	54	215	96	0	2	64	18	1	440	39	4	13	0	55	32	117	51	3	0	7	0	0	0	1	251	1	11	131	0	1	0	0	4	39	39	72	0	0	1	0	0	0	0	0	0	1	3	0	0	0	0	2	13	1	11	0	2	0	0	5	6	0	0	0	0	4	1	0	0	2	3	0	0	1	0	0	1	0	0	3	0	0	0	3	0	5	1	4	0	0	0	0	0	0	0	1	1	1	0	0	0	0	0
.......................................................................CAGCCATTTTGACGAGTT............................	18	2	1149	44	57	61	147	29	74	32	18	5	23	13	18	11	6	3	87	7	1	10	5	3	2	1	2	0	6	42	6	6	30	14	7	2	19	0	0	3	1	0	8	2	16	5	39	1	2	2	10	5	3	1	9	5	0	0	5	7	2	15	2	1	0	14	4	6	5	0	2	3	3	0	3	11	5	7	5	0	3	16	0	0	8	4	10	11	2	12	0	1	0	4	0	0	0	27	5	0	0	0	5	14	0	0	1	0	1	0	0	1	0	12	0	1	0	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................CAGCCATTTTGACGAGTTT...........................	19	2	44	2	0	1	6	1	8	0	0	0	4	0	0	0	0	0	2	0	0	0	0	0	1	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	3	0	1	0	0	0	0	0	0	0	0	0	3	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................AGCCATTTTGACGAGTTT...........................	18	2	74	0	6	2	5	0	3	2	1	0	12	0	0	1	1	0	5	0	1	1	0	0	1	0	0	0	0	1	0	1	3	0	0	0	0	0	0	0	1	0	0	0	1	3	4	1	1	0	0	0	1	0	3	0	0	0	0	0	0	1	0	1	0	0	0	2	0	0	0	0	0	0	0	1	0	4	1	0	1	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................AGCCATTTTGACGAGTTTG..........................	19	2	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................TGGGTAAAGAAATCTCAACATTGAACG.	27	1	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Antisense strand

	Size	Blast	Total	V065 dcr-2[L811fsX], ovary, AGO1IP	V098 dcr-2[L811fsX] ovary	V066 r2d2[1], ovary, AGO1IP	V063 CS,ovary,AGO1IP	V088 r2d2[1] ovary total RNA	V064 ago2[414], ovary, AGO1IP	V097 CS ovary	V076 ML-DmBG3-C2	V089 ago2[414] ovary total RNA	SRR032095 AGO1 IP dcr2 knockdown	V081 embryo 2-6hr	V082 embryo 14-24hr	V067 S2-NP	SRR031696_2152	SRR014281 Ovary_rep1 wK(fem)_x_w1118(male)_F1	SRR014274 Ovary_rep1 LK(fem)_x_Har(male)_F1	V024 kc167 cell	V026 1182-4H cell	V019 GM2 cell	GSM379057 Krimp Mutant	SRR014280 Ovary_rep1 w1118_P	GSM280088 S2cell (AGO1IP)	GSM379065 Zuc Heterozygote	GSM379064 Vasa Mutant	GSM424740 S2 pKF63 stably transfected	S33 KC-48 #2	SRR014276 Ovary_rep1 NA(fem)_x_Har(male)_F1	S32 s2+48 #2	GSM379063 Vasa Heterozygote	SRR014275 Ovary_rep1 LK_P	V033 CMEW1 Cl.8+ cell	SRR014271 Embryo_0-2hrs rep1_w1118_0_2hr_Emb	V011 Sg4	SRR029028 untreated (mock)	V021 ML-DmD21 cell	SRR001345 ago2 non-beta-eliminated	V018 Kc167 cell	V008 S2-DRSC	SRR031703 Total small RNAs from r2d2 homozygous flies	SRR014279 Ovary_rep1 w1118(fem)_x_wK(male)_F1	V009 CMEL1	SRR014278 Ovary_rep1 w1118(fem)_x_Har(male)_F1	SRR014270 Embryo_0-2hrs rep1_NA_0_2hr_Emb	SRR014277 Ovary_rep1 NA_P	GSM379056 Krimp Heterozygote	SRR029633 total small RNAs from hen1 homozygous flies	SRR014282 Ovary_rep1 wK_P	GSM379066 Zuc Mutant	GSM379060 SpnE Heterozygote	V025 S2R+ cell	V090 CS  male total RNA	SRR014269 Embryo_0-2hrs rep1_LK_0_2hr_Emb	GSM379052 Aub Heterozygote	SRR031701 Total small RNAs from r2d2 heterozygous flies	SRR014272 Embryo_0-2hrs rep1_wK_0_2hr_Emb	V028 CMEW1 Cl.8+ cell	S31 s2+48 #1	GSM379062 Squ Mutant	SRR014273 Ovary_rep1_Har_P	SRR031692 Total small RNAs from Oregon R	SRR031697 Total small RNAs from dcr-2 heterozygous flies	GSM379051 Armi Mutant	SRR029030 dcr-2 knockdown	GSM379061 Squ Heterozygote	SRR031699 Total small RNAs from dcr-2 homozygous flies	V083 male, one day	S29 male head #1	V002 IR+	SRR032094 ago2 knockdown	GSM379058	SRR001349 heterozygous dcr-2_untreated	V020 S2R+ cell	OSS6 Drosophila OSS cell small RNA libraries, rep2	V084 female, one day	SRR029031 loqs-ORF knockdown	V001 IR-	S30 s2-48 #2	GSM379067 SpnE Mutant	OSS2 Drosophila OSS cell small RNA libraries, rep1	GSM379054 Flam Heterozygote	V010 MLDmD20c5	SRR029032 r2d2 knockdown	GSM379050 Armi Heterozygote	OSS7 Drosophila OSS cell small RNA libraries, rep3	OSS8 Drosophila OSS cell small RNA libraries, rep4	V075 ML-DmBG1-C1	SRR032093 ago1 knockdown	SRR001338 IR non-beta-eliminated	GSM379053 Aub Mutant	S35 KC+48 #2	S15 S2 -48 #1	GSM280084 loqs-/- ovaries (18-29nt)	SRR001664 homozygous dcr-2_untreated	S16 KC -48 #1	GSM280083 dcr-2-/- ovaries (18-29nt)	SRR029033 lacZ knockdown	GSM343287 Drosophila Toll 10b mutant embryos	SRR001347 ago2 untreated	S34 KC+48 #1	SRR014268 Embryo_0-2hrs rep1_HAR_0_2hr_Emb	SRR029029 dcr-1 knockdown	GSM379059	SRR001344 dcr-2 beta-eliminated	S24 male body #2	S12 6-10h #1 (10)	S28 0-2d pupae	S9 0-1h #3 (7)	S10 2-6h #1 (8)	S13 6-10h #2 (11)	S27 0-1d Pupae (w)	GSM280082 WT ovaries (18-29nt)	S14 0-1hr #1 (A)	GSM280085 WT testes (18-24nt)	GSM379055 Flam Mutant	S22 female head #1	S23 female body #2	S2 female head	S8 S2-48,+48, KC-48, +48 mix	S19 2-4day pupae #1	SRR001341 WT_males non-beta-eliminated	SRR001339 WT_females non-beta-eliminated	SRR001343 dcr-2 non-beta-eliminated	GSM424739 S2 parental	SRR010954 Aub trans-heterozygotes, oxidized	S21 disk #2	S5 imaginal disc	SRR031704 2-O-methylated small RNAs from r2d2 homozygous flies	S7 12-24hr embryo	S11 2-6h #2 (9)	S1 male head	SRR010953 Aub heterozygotes, oxidized	SRR001348 ago2 oxidized	SRR010959 Ago3 IP in heterozygotes	S4 female body	GSM239052 S2 cells, non beta-eliminated	S18 3rd inster #2	GSM239041 fly heads, non beta-eliminated	SRR032092 mock oxidized	SRR031698 2-O-methylated small RNAs from dcr-2 heterozygous flies	GSM467729 WT	SRR031700 2-O-methylated small RNAs from dcr-2 homozygous flies	GSM424741 S2 pKF63 transiently transfected	S17 3rd inster #1	GSM180337 tissue culture cells (S2 only)	SRR031702 2-O-methylated small RNAs from r2d2 heterozygous flies	SRR001346 ago2 beta-eliminated	GSM467730 r2d2	GSM180328 adult heads (female heads, male heads)	S3 male body	SRR032096 AGO2 IP dcr2 knockdown	S20 2-4day pupae#2	SRR001337 WT_females beta-eliminated	GSM280087 S2cell (AGO2IP)	S25 1st instar #1	GSM180333 late embryo (12-24)	SRR010956 Piwi IP in Ago3 heterozygotes	SRR031693_4	GSM180332 mid embryo (6-10)	GSM180335 imaginal discs	SRR010955 Aub IP in Ago3 heterozygotes	GSM180330 very early embryo (0-1)	GSM180331 early embryo (2-6)	S26 1st instar #2	GSM467731 loq	GSM180329 adult bodies (female bodies, male bodies)	SRR010958 Piwi IP in Ago3 trans-heterozygotes	GSM180334 larvae: 1st instar and 3rd instars	GSM246084 D. melanogaster adult male heads 454	GSM180336 pupae: 0-1 day, 0-2 day, 2-4 day	SRR001340 IR beta-eliminated	SRR010960 wt, oxidized	SRR023400 total RNA extracted from P19 cells	SRR023399 RNA bound by P19 protein	SRR023197 RNA Library from S2 control cells	GSM154618 23-29 nucleotide RNAs from Drosophila melanogaster ovaries	SRR001342 WT_males beta-eliminated	SRR010951 Ago3 heterozygotes, oxidized	SRR010952 Ago3 trans-heterozygotes, oxidized	GSM239050 fly heads, beta-eliminated	GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006)	GSM154621 piRNAs associated with Ago3 from Drosophila melanogaster ovaries	SRR010957 Aub IP in Ago3 trans-heterozygotes	SRR023407 RNA bound by NLS-P19 protein	GSM280086 WT ovaries (AGO2IP)	GSM239051 S2 cells, beta-eliminated	SRR023402 total RNA extracted from NLS-P19 cells	GSM266765 Siomi Lab fruit fly S2 AGO2 Cloned Sequences v1.0	GSM154622 piRNAs associated with Aubergine from Drosophila melanogaster ovaries	GSM154620 piRNAs associated with Piwi from Drosophila melanogaster ovaries
.........CACGTCTTTCTTATACCA..........................................................................................	18	1	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............TCTTTCTTATACCATGTC......................................................................................	18	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................ACAGCCATTTTGACGAGTTT...........................	20	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0