Page generated on Fri Oct 15 12:01:08 2010

dme-mir-2a-1




dme-mir-2a-1

View dme-mir-2a-1 on miRBase
miRNALengthPerfect(sense)Perfect(antisense)5' moR5'armLoop3'arm3' moROtherLoop SizeMatureStar
dme-mir-2a-112512275649786407716212234012711201223401240771


ATAAAACTATATTGGCGCATTCATGCTGGGCTCTCAAAGTGGTTGTGAAATGCATTTCCGCTTTGCGCGGCATATCACAGCCAGCTTTGATGAGCTTAGCGTAATGTATCGTACATCAAATCATA
........................((((((((((((((((((((((((.((((.....(((.....))))))).))))))))).)))))).))))))))).........................-41.90
........ 0.00
Sense strand
                                                                                                                             SizeBlastTotalV098 dcr-2[L811fsX] ovaryV064 ago2[414], ovary, AGO1IPSRR032095 AGO1 IP dcr2 knockdown V065 dcr-2[L811fsX], ovary, AGO1IPV082 embryo 14-24hrV063 CS,ovary,AGO1IPV018 Kc167 cellV088 r2d2[1] ovary total RNA  V097 CS ovaryV066 r2d2[1], ovary, AGO1IPV011 Sg4V076 ML-DmBG3-C2V021 ML-DmD21 cellV026 1182-4H cellV008 S2-DRSCV009 CMEL1V067 S2-NPGSM424740 S2 pKF63 stably transfected V081 embryo 2-6hrV020 S2R+ cellV033 CMEW1 Cl.8+ cellV089 ago2[414] ovary total RNA  GSM280088 S2cell (AGO1IP) V024 kc167 cellSRR014271 Embryo_0-2hrs rep1_w1118_0_2hr_Emb V025 S2R+ cellV001 IR-SRR029031 loqs-ORF knockdown V002 IR+V019 GM2 cellSRR014281 Ovary_rep1 wK(fem)_x_w1118(male)_F1 SRR014278 Ovary_rep1 w1118(fem)_x_Har(male)_F1 S33 KC-48 #2SRR014279 Ovary_rep1 w1118(fem)_x_wK(male)_F1 SRR014280 Ovary_rep1 w1118_P SRR014276 Ovary_rep1 NA(fem)_x_Har(male)_F1 V028 CMEW1 Cl.8+ cellSRR014268 Embryo_0-2hrs rep1_HAR_0_2hr_Emb SRR014269 Embryo_0-2hrs rep1_LK_0_2hr_Emb SRR014275 Ovary_rep1 LK_P SRR031696_2152 SRR014274 Ovary_rep1 LK(fem)_x_Har(male)_F1 GSM379052 Aub Heterozygote GSM424739 S2 parental GSM379063 Vasa Heterozygote SRR029028 untreated (mock) GSM379057 Krimp Mutant V010 MLDmD20c5V090 CS  male total RNA  SRR014277 Ovary_rep1 NA_P S32 s2+48 #2GSM379066 Zuc Mutant SRR014273 Ovary_rep1_Har_P GSM379054 Flam Heterozygote GSM379050 Armi Heterozygote GSM379064 Vasa Mutant GSM379065 Zuc Heterozygote SRR014270 Embryo_0-2hrs rep1_NA_0_2hr_Emb V083 male, one daySRR029032 r2d2 knockdown SRR001345 ago2 non-beta-eliminated GSM379051 Armi Mutant SRR014272 Embryo_0-2hrs rep1_wK_0_2hr_Emb SRR029030 dcr-2 knockdown SRR029033 lacZ knockdown SRR014282 Ovary_rep1 wK_P GSM379060 SpnE Heterozygote S29 male head #1V084 female, one dayGSM379058 SRR029029 dcr-1 knockdown GSM379056 Krimp Heterozygote S31 s2+48 #1S35 KC+48 #2SRR032094 ago2 knockdown GSM379053 Aub Mutant GSM379061 Squ Heterozygote GSM379062 Squ Mutant SRR031703 Total small RNAs from r2d2 homozygous flies S34 KC+48 #1OSS6 Drosophila OSS cell small RNA libraries, rep2 SRR029633 total small RNAs from hen1 homozygous flies OSS2 Drosophila OSS cell small RNA libraries, rep1 SRR031699 Total small RNAs from dcr-2 homozygous flies S30 s2-48 #2SRR031697 Total small RNAs from dcr-2 heterozygous flies V075 ML-DmBG1-C1SRR031701 Total small RNAs from r2d2 heterozygous flies OSS7 Drosophila OSS cell small RNA libraries, rep3 SRR031692 Total small RNAs from Oregon R GSM379067 SpnE Mutant GSM280084 loqs-/- ovaries (18-29nt) GSM379055 Flam Mutant S13 6-10h #2 (11)SRR032093 ago1 knockdown S12 6-10h #1 (10)S16 KC -48 #1OSS8 Drosophila OSS cell small RNA libraries, rep4 SRR001338 IR non-beta-eliminated GSM280085 WT testes (18-24nt) S22 female head #1SRR001344 dcr-2 beta-eliminated GSM424741 S2 pKF63 transiently transfected SRR001347 ago2 untreated GSM280082 WT ovaries (18-29nt) S28 0-2d pupaeGSM280083 dcr-2-/- ovaries (18-29nt) SRR001664 homozygous dcr-2_untreated SRR001341 WT_males non-beta-eliminated SRR001339 WT_females non-beta-eliminated GSM379059 S24 male body #2SRR001349 heterozygous dcr-2_untreated S8 S2-48,+48, KC-48, +48 mixS10 2-6h #1 (8)SRR001343 dcr-2 non-beta-eliminated GSM343287 Drosophila Toll 10b mutant embryos S9 0-1h #3 (7)S27 0-1d Pupae (w)S15 S2 -48 #1S1 male headSRR032096 AGO2 IP dcr2 knockdownS2 female headSRR031704 2-O-methylated small RNAs from r2d2 homozygous flies SRR010954 Aub trans-heterozygotes, oxidized S14 0-1hr #1 (A)S7 12-24hr embryoGSM239052 S2 cells, non beta-eliminated S23 female body #2S11 2-6h #2 (9)S21 disk #2SRR010953 Aub heterozygotes, oxidized S19 2-4day pupae #1S5 imaginal discGSM280087 S2cell (AGO2IP) GSM467730 r2d2 SRR032092 mock oxidized GSM467729 WT SRR010959 Ago3 IP in heterozygotes S26 1st instar #2GSM180328 adult heads (female heads, male heads) SRR001348 ago2 oxidized SRR031700 2-O-methylated small RNAs from dcr-2 homozygous flies S25 1st instar #1SRR031698 2-O-methylated small RNAs from dcr-2 heterozygous flies SRR001346 ago2 beta-eliminated SRR001337 WT_females beta-eliminated S17 3rd inster #1GSM180333 late embryo (12-24) S4 female bodyS3 male bodyGSM180332 mid embryo (6-10) GSM239041 fly heads, non beta-eliminated GSM467731 loq S20 2-4day pupae#2GSM180337 tissue culture cells (S2 only) GSM180331 early embryo (2-6) S18 3rd inster #2SRR010955 Aub IP in Ago3 heterozygotes SRR023400 total RNA extracted from P19 cells SRR010956 Piwi IP in Ago3 heterozygotes GSM180335 imaginal discs SRR031702 2-O-methylated small RNAs from r2d2 heterozygous flies SRR031693_4 SRR010958 Piwi IP in Ago3 trans-heterozygotes GSM180329 adult bodies (female bodies, male bodies) GSM180330 very early embryo (0-1) GSM180336 pupae: 0-1 day, 0-2 day, 2-4 day GSM180334 larvae: 1st instar and 3rd instars SRR023399 RNA bound by P19 protein SRR001340 IR beta-eliminated SRR010960 wt, oxidized GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006) GSM246084 D. melanogaster adult male heads 454 GSM239051 S2 cells, beta-eliminated SRR001342 WT_males beta-eliminated SRR010952 Ago3 trans-heterozygotes, oxidized SRR023197 RNA Library from S2 control cells GSM154618 23-29 nucleotide RNAs from Drosophila melanogaster ovaries SRR010957 Aub IP in Ago3 trans-heterozygotes SRR023407 RNA bound by NLS-P19 protein SRR010951 Ago3 heterozygotes, oxidized GSM239050 fly heads, beta-eliminated GSM280086 WT ovaries (AGO2IP) GSM154621 piRNAs associated with Ago3 from Drosophila melanogaster ovaries GSM154620 piRNAs associated with Piwi from Drosophila melanogaster ovaries SRR023402 total RNA extracted from NLS-P19 cells GSM154622 piRNAs associated with Aubergine from Drosophila melanogaster ovaries GSM266765 Siomi Lab fruit fly S2 AGO2 Cloned Sequences v1.0
.....ACTATATTGGCGCATTCATGCTGGG...............................................................................................2511000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
......CTATATTGGCGCATTCATGCTGGG...............................................................................................24110000000000000000100100000010010400000000000000000000000000000000000000000000000000000000000000200000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.......TATATTGGCGCATTCATGCTGGG...............................................................................................23112100000010000000000100001000000000002000100000001000000000000100000000100000000000000000000100000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000
........ATATTGGCGCATTCATGCTGGG...............................................................................................221461000001100102122127110001300000000000100000001000001000000100000003000000000000000100000000000000000000000000000000100000000000000000000000000000000000000000000010000000000000000000000000000
.........TATTGGCGCATTCATGCTGGG...............................................................................................2111180000509020172243001373001002032001100013203101020001222022500040000300001000000130100001000000300000000000000000000100001000000001000000000000000000000000000000010000000000000000000000000000
..........ATTGGCGCATTCATGCTGGG...............................................................................................20127760006013830710155372029133001021011603013311101104034088027416010080000600300260205050000000000303601200000001030001000200400000001000020010000001000000001100000000000002000000000000000000000000
...........TTGGCGCATTCATGCTGGGC..............................................................................................2011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000
...........TTGGCGCATTCATGCTGGG...............................................................................................191263200060143204491511003013500175701444812011111023000730060320123000000000001014300000501004000000007110000001000000001001100316000000011400000000000000000000000010000000000000020000000002200000000
............TGGCGCATTCATGCTGGG...............................................................................................18158000030000014420000832000020001000000120000000000005000000000000000000000320000020100300000000100000000000400000001000020000000000000000000000001000000000000000000000000000000000000000000000
..........................TGGGCTCTCAAAGTGGTTGTGA.............................................................................2211000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................GCTCTCAAAGTGGTTGTGAAAT..........................................................................2213000000000000000000100000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000
.............................GCTCTCAAAGTGGTTGTGAAA...........................................................................2111000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000
..............................CTCTCAAAGTGGTTGTGA.............................................................................18127380904030111501020011160010344300370037510001002000120101001200000000010201130000582119162000313200008431200100001000001000000010200200002000003000000110000000000000000000100000000000001000000000000
..............................CTCTCAAAGTGGTTGTGAAATG.........................................................................22131618175477931748474929712521517384541974101422302092154143715475350354142331754336044702823216923311237318126544863353174291315343882583001599514260338106164334216217223457188135535564921274553629576872171931841881715096763161311375187335039414531347992636157516109603501372243310251361372143284114164741008319125323509937740702042749210150002566016001041810801665820035300501001000011000000
..............................CTCTCAAAGTGGTTGTGAAA...........................................................................20128016532662040164957121152623291026272172051341414483257133167161651196225311012115265531584240054108216001781150551155395830201434372148136101314130205613642344010300234030002014350935616263206300413024301800000100300101020110020001210000000202001000000000000
..............................CTCTCAAAGTGGTTGTGAAAT..........................................................................211422510749263827261486251262342811190803725631375681636141178110298141673210518522366393341157558008317722011817781943611247015501301031991769651109680702071162402110933100033912000311242248132911622121040718220111620045230014021900332030030205002370001000000000000000000000
..............................CTCTCAAAGTGGTTGTGAAATGCA.......................................................................2412000000002000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
..............................CTCTCAAAGTGGTTGTGAA............................................................................1911099459371632222532003323050272080910370531332424411415001121102554221121611000115632111017324594000235581347012003434200711292842003001110110000118501118031200211150102000000010200002000000000000220000000100001000000000000
..............................CTCTCAAAGTGGTTGTGAAATGC........................................................................23123200002010010000000212000021000000000001000000000000000000000000000000000200010000002010000000010000000000000000000000000000000100000000000000000000000000000000000000000000000000000000000000
..............................CTCTCAAAGTGGTTGTGAAATGCATTTC...................................................................2811000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
..............................CTCTCAAAGTGGTTGTGAAATGCAT......................................................................2511000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000
...............................TCTCAAAGTGGTTGTGAAAT..........................................................................20140201000012000010000300000000010002000302000000100000000000100000000000000100000012010000012000060100000000000000000001010000100000000001000000000000000000000100000000000000000000000000000000
...............................TCTCAAAGTGGTTGTGAA............................................................................1819000000000000000000100000001000000000000000000000000000000000000000000000000000001110000000000000020000000000000000000000010100000000000000000000000000000000000000000000000000000000000000000
...............................TCTCAAAGTGGTTGTGAAATGC........................................................................2213001000100000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...............................TCTCAAAGTGGTTGTGAAATG.........................................................................2113501184354131251300243025641110318200271343211061012230241504222040121281200231470005200103621001200020050100000301000001071000107113000000100090100000040500000000001102000120010000000000000000000000
...............................TCTCAAAGTGGTTGTGAAA...........................................................................19120102101000000000010000000030000000000000000100000000000000000000100000000000000001000000010000050000000000000000000100000000000000000001000000000000000000000000000000000000000000000000000000
................................CTCAAAGTGGTTGTGAAATG.........................................................................201801030000000012021103001010110004010030001040006110000100000000000000000000040000000100100000000150000000000000000000200000020100000000001000000060500000000000000000020000000000000000000000000
................................CTCAAAGTGGTTGTGAAAT..........................................................................1919000000000000000000200000000000000000000000000000000000000000000000000000000000000000000000000070000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
................................CTCAAAGTGGTTGTGAAATGC........................................................................2111000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000
................................CTCAAAGTGGTTGTGAAA...........................................................................18120000000000001000000000000000000402000001000000000000000000007000000001000000000001010000010000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.................................TCAAAGTGGTTGTGAAATG.........................................................................19283520001012201011010202002202200101001300101000200003020012100010000000000020001003001010001000060410020000000000100000002000000001000000000000020200001000000000000120000000000000000000000000
.................................TCAAAGTGGTTGTGAAAT..........................................................................18224100000000000000010101000000100002060011100001000000010010100000000000000000000001000100000000010100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.................................TCAAAGTGGTTGTGAAATGC........................................................................2011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000
..................................CAAAGTGGTTGTGAAATG.........................................................................183842000510230011000001311003800100010001100202300300011000110000010500000100010010001000000000001170100000000000001000000001000000000000000000000001100000000000000000010000000000000000000000000
..................................CAAAGTGGTTGTGAAATGCATTTCCGC................................................................2711000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...................................................GCATTTCCGCTTTGCGCGGCATATCAC...............................................2711000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
....................................................CATTTCCGCTTTGCGCGGCA.....................................................20130000000000000000000200000006030000000000000000000000000000000000000000100000000400300010000000300001000000000000000200000000001100000020000000000000000000000000000000000000000000000000000000
....................................................CATTTCCGCTTTGCGCGG.......................................................1811000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.....................................................ATTTCCGCTTTGCGCGGCA.....................................................1911000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
......................................................TTTCCGCTTTGCGCGGCAT....................................................1915000000000000000000000000000000000000000000000000000000000000000000000000000000000010000010000000020000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000
......................................................TTTCCGCTTTGCGCGGCA.....................................................18223000000000000000000000000000000000000000000000000000000000000000000000000000000004080000060000100040000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.......................................................TTCCGCTTTGCGCGGCAT....................................................1811000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.......................................................TTCCGCTTTGCGCGGCATA...................................................1911000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
..........................................................CGCTTTGCGCGGCATATCACAGCCAGCTT......................................2911000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...................................................................CGGCATATCACAGCCAGCTTTGATGAGCT.............................2911001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.....................................................................GCATATCACAGCCAGCTTTGATGAGCT.............................2721000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
......................................................................CATATCACAGCCAGCTTTGAT..................................2121000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
......................................................................CATATCACAGCCAGCTTTGATGAGCT.............................2624100000000000000000000000000001010000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000
......................................................................CATATCACAGCCAGCTTTGATGAG...............................2422000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000100000000000000000000000000000000000000000000000
......................................................................CATATCACAGCCAGCTTTGATGAGC..............................2523000000000011000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGATGAG...............................23216700010101001010200012200000000100220010210010000000027001000000000001260060139000001110011400010000701100100000000001412300400101002000010000100000000000010000000000000000010000000000000010000000
.......................................................................ATATCACAGCCAGCTTTGATGAGC..............................24210401001000101021210001200000001200170010111001000000015011000006000000060000540000130010710010000001000020000400000100000130000000000000000000000000000000010000000000001000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGATGAGCT.............................252468853219311832671137240171192010442617254201202416101211063580130222201000141911002170311217203191002201112013220010000700000300300022000002110020200000000000000100000000100010000000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGAT..................................2023000000000000000000000000000000000000000000000000001000000000000000000000000000010000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGATGA................................2227500000000000000001000100000000000120000001100010000030000000000000000170000620000020000200110000000510020000600000000000001000000021001010000003000000000001000000000000000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGA...................................1944000000100000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000010000001000000000000000000000000000000000000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGATGAGCTT............................2619000000000000000110011001000000000000000000000000100000000000000000000100000000000000010000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.......................................................................ATATCACAGCCAGCTTTGATG.................................21247000000000001010000000000000000007000000000000000006000000000000000010000030000031130000010000000300000010400000000200000301000100010200000000000000000000000000000000100000000000000000000000
........................................................................TATCACAGCCAGCTTTGATGAG...............................22249955769066420691892651355895382886734456253211811511082132929241277542032328011818022327106386619902198786327474329828858971122542441317021104240021701468265341642854210152021836394912632282147638248279107717481808324681875330521402658256719507692083455522552640143515092026255997074162330198519019166927017402041913139216527249559219095293336746593183684115245111524139299513132185483219816773472637550165695049795445829985106951375137510791219671174822261099850183614599637476943312120103758586837449933112265316427007056956323024776831831176773117155131971341732924914416666260191741082236127871195936864283393337716100000
........................................................................TATCACAGCCAGCTTTGATGAGCTTAGCGT.......................3011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGATGAGCTTAGCG........................2911000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGATG.................................20340143112555661491516029484386230611102542591707334111040251132231154035018629442335540353320318242362552955191448878746104101273109661614385160378584323415358432116314213453266215119347625111419643112656863370169873380060873715526620359155927107829710634052462699202422229517362343737856311882438471166633613529736633917118897383332399971841834251001613141062919203303638671407135428267260501191001616963740515001120000000
........................................................................TATCACAGCCAGCTTTGATGA................................21211393735618217264906646643328930619979815965016322776782249351284773079630871181433921611236971615216520928476138108142170723320527541129275256487142171457417911622211424019210285316697512579333235781494903441283921622967221852412369120500817742229342122251952217820667932501959223378656146712229316729541771130220851711101112736895381081493493112111089147631010441358144687355181955022121243714130143710438416729123676809295832151204974591111547938921602241245259255943025433818293717161921211351107000050000000
........................................................................TATCACAGCCAGCTTTGATGAGCTTAGC.........................2814000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000000000000000000000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGATGAGCTTAG..........................27115020100102000000000000000000000200120110000000000000000100000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGATGAGC..............................2324251087835444016344909381839721512157604697352321155680622144131232658120427255841452496169054628173911998972320364891441126616971549627721775121402707264897850263334275814904491121438101301946118525588127782149272212277147930052206171218241583141515303171253431517881981216720042787972176020311537515949161364186012161040116539614811151749711965268219265998003893129289878110801120442140014152386455171555763231170844410734075101738173865557153990882081554965984661811227617767460312378266351215541802179549443199510772708578378355137974510665234711010329891778581920713740312430117823839103343061000
........................................................................TATCACAGCCAGCTTTGATGAGCTTAGCGTAA.....................3211000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGATGAGCTTA...........................261238724512561861680498313260593239594232140363618690217817515162280282427227799371138140333125211738171113512611003153300141501213013010201412502013253121772000000000011010004120000011000000000010000605000000000000000200100000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGA...................................18596432219100927123312212131510164557452171401640779312162933493860587448030208544572457527012719233641253917105067807697610261312315320222373211132043823834143491614224912216423263211310028264453255101146531072748312262090201242115807400003101156428302000000000002003118000000049000010200000000000
........................................................................TATCACAGCCAGCTTTGAT..................................193110252654206607477526153732701671542852673034485490220571252623742392485366318183038233200167718397630311925126191826244871126417221446213312201471445723616350862043132261135912517304180109618712271514012015745139401043332381071212391241713324111063194848251327536116333212205671952000960481161000120132101002005010015900000021402004000060000000
........................................................................TATCACAGCCAGCTTTGATGAGCTT............................251243140138917962183915478520546501213359317718410411454815849361170294597096721402244505539284398555637620933917134932711033216116012953241181317832142440520192938189541715938060122313641027142823221339295783165577610747689132052279630212585484791976276563013816185561130537175945155469244799075200922272221012357267915991291433274452063143153160531403977261033825251742330173991381727357823013918911100131192238194771033872512534343160002331101100000200000000101000
........................................................................TATCACAGCCAGCTTTGATGAGCTTAGCGTA......................3111000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000
........................................................................TATCACAGCCAGCTTTGATGAGCT.............................242622139543336829102022529423328930391020395715863822700420997019455016408218934513042116530110821310572111033840519181368978811025571074046645856795687446702581972583216758032899665544645828985607995949959018465352963940154506142207751910426161467424343543943340281572658041561134083806336769347573471535328373653081335568260981676733888232622341719785296632680641612835723917183552219988997347155921967024395206561018064787767791575595348675659001691868155877630010302880192499053765793664163501047065722002684159731875359297049622651119611963684957268117842673746357931535140835118024926046564131025867713281257699289534497709100420277207374127288169243136255326174834472332210174167159109321791511156299343854411431111281514231100252110
.........................................................................ATCACAGCCAGCTTTGATGAGCTTA...........................25110201000011010000000000000000000000000000000000000000001000000000000000000000000000000100000000000000000000000000000000000000000000000000200000000000000000000000000000000000000000000000000000
.........................................................................ATCACAGCCAGCTTTGATGAGCT.............................23225398166692815567271649733535522762558558289546332566332191985633543827522199621116724730420629565134221182213102118141102155961911377501232461151091329192106609774691067824922782864616117947595212786653679751373395518228356447435448594628543513515176631539221618507191019119981081591018941603027423503250121100003110010010200212000100001000000000000000000000
.........................................................................ATCACAGCCAGCTTTGATGA................................2026580482020010051052000100110010010219001020181010000121140000100004300700067001201358000583421812815033422000100752201413050102330730284544902200004000120302002033030001100100010000000000100000000000000000000
.........................................................................ATCACAGCCAGCTTTGATG.................................193270313200100003201100001000000002041600000001100000030151000000001000000360000411300011361241801302541000009650700003000007000001101070100135000000000000100000101100110000100000000001000000100000000000
.........................................................................ATCACAGCCAGCTTTGATGAGCTT............................241130851191092046187627441363221623742313609301017414472685710121001071017985271051447145563652147002822110165982200241016043107040411000050000000000000100000000040000000000000000000100000000000100100000000000000000000000000000000
.........................................................................ATCACAGCCAGCTTTGATGAGC..............................22216853511141718275391989714582614674340874843339022711945474160314121041623029633435023559137713104191013146176253303321215264272211211521652453532332032014710440222340244100201211000000000010002100011300101102000000000100000000000000000000
.........................................................................ATCACAGCCAGCTTTGAT..................................18342004000000000011000000000000010023000000020000000001000000000000000010000000000201210110321000000002000010000000001000000000130000004000000000000000000000000000000000000000000000000000000000
.........................................................................ATCACAGCCAGCTTTGATGAG...............................21214391461811427887102391646233647113517133424180165430241433802029010204930211214181322313031500004363123102023321128311232112221330483932388234423219011515012062102563180520118200002017070340031111310101000001000001000000000000000000000000
..........................................................................TCACAGCCAGCTTTGATG.................................18341802319072812143721792401115104010305912363000261238952010021068902000107113520000674320030341010332412122181722310904913550005315308127592210542223371818310926175405620141030155641513551241912640508050140131110802100813035210000500310000010302001000000000000
..........................................................................TCACAGCCAGCTTTGATGAGCT.............................22241340352691603185432208401993245471614032114330310275611578811663892394946371191688170973734594398507715038518592661456003552826815993691343986295582981945062341973435628966372452002023452218382261924549421143140621496270371768823441709852270829497187941793331087165391446417996186791654518287176341556120392173292592623270159792403424673259401200012014581110634119711409512598889410421150781107668059615109347795236074222535623547685375378465532046553755055621486332325427198247601287485717511288461968253752430419894134341728122812296828102685277711222105131098126715105782054906548275394539974669565927075421458180588141924421553801433181934433412532022071991275965200917117343299195453574382531377866013121230909901600000
..........................................................................TCACAGCCAGCTTTGATGAGCTTAGCGT.......................2811000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000
..........................................................................TCACAGCCAGCTTTGATGAG...............................20257814342781337786120600873147127244104067597549223361137172521237452014279361702019111512566726686703313351161641633230944127173671357761332240907646510745597810962077787846239229223279175730230344394261746219258132354908694892096369149314571402531146219596693812442639191377269199943941232313437147141053122241274331334382431155410435421701619102264107275010461682412623551232293547604216502216264041951191045131170541321803562344020107724000100
..........................................................................TCACAGCCAGCTTTGATGAGCTTA...........................241153851648633432413451184072521501823013301019713311942903314101145755941132860190892183496861231406817745305445121714340100012330113183420110010010020112212000001001101010100010920910000100000001002200100000000000000000000000000000000
..........................................................................TCACAGCCAGCTTTGATGAGCTT............................231174038783181591986835055712389959039473792285357504373398249028496381161661432389594924352299346942441428308543345683312525852195041386276383729471949112573043071639476690147647812422240506414369905550506443384723799136976454727109213843342991214432852289323236135688345291214791296951782301081433194565451777812092025326985019543517041992184271313133722661151102924310243356011825103906633127171304048421413429324246313212102100200000000001100000000
..........................................................................TCACAGCCAGCTTTGATGAGCTTAGC.........................2613000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000201000000000000000000000000000000000000000000000000000
..........................................................................TCACAGCCAGCTTTGATGAGCTTAGCG........................2711000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000
..........................................................................TCACAGCCAGCTTTGATGAGC..............................21211476621071682671176532890947308339277714288121946892018594134366782811974875819350381154372912512575613404137437930218733036415082676736121438821657131323254663541613416203222939925346639029027622521977910743805904281129279257308213225597724417301094762339851818601078114594112262128120914523356210166151231641203251981244404042402034150829036953013128811446291891824968331436616844512661752013846386150661831123492451479363705812561066201061645878476825551728575320117272025272441362119233201510530447163220601117000000
..........................................................................TCACAGCCAGCTTTGATGA................................1922016242215470315441121732577741291447512044212862809319405039811935562432240515092108838517412412491413310960801511012189611510265901280413844211411061187055857419428678248333124539494143758544045871062403463463122422983191688112982721302472573399534274943276623013403604804219342635702301733021121500130100141000000161901022000062000000
..........................................................................TCACAGCCAGCTTTGATGAGCTTAG..........................25117000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000208000000000000000700000000000000000000000000000000000
..........................................................................TCACAGCCAGCTTTGATGAGCTTAGCGTAA.....................3011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000
...........................................................................CACAGCCAGCTTTGATGAGCTT............................221434165745610126431356424103701141012603110070732028040600006237201210012400100701040220100130101023052000011000012000001000000001000000010000000000001803000000000000010100000000000000000000000000000000000
...........................................................................CACAGCCAGCTTTGATGAGCT.............................212756824628776021514860214679901181159411958944524889501105728161384536469838582880783120332244513031626191471610315193232452411101217122459671591010114157277732974813474449111810134292083148923695462060611515239151728363101891361117175818120170334072303324920102142131314130010410110000000100000100000000000000000000
...........................................................................CACAGCCAGCTTTGATGAGC..............................2025525141265217212111144156400920135400110061119244104476202612231105121011291406217116041911162111213369180588001001071063010112221220051100200060020000000000111001010000100000000000000000000000200000000000000
...........................................................................CACAGCCAGCTTTGATGAG...............................1923550270210220311117310110000000012010102022030240121001703000001081531305100002200104312126261220587010200072544010301770100000100302001010300000201010011100000001000000000000000000000000000000000000
...........................................................................CACAGCCAGCTTTGATGA................................182170008020200000003020100000010001030000000018000000000000000000050000001300001030016031607470538000000022049010001440300010010001131101004000000020000001000000000000000000000000000000000000000000
...........................................................................CACAGCCAGCTTTGATGAGCTTAG..........................2411000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000
...........................................................................CACAGCCAGCTTTGATGAGCTTA...........................23118000000000000002030000000000000000000000000000000000000000000000000000090000000000000000000000000000000000000000000000000000000000000000202000000000000000000000000000000000000000000000000000
............................................................................ACAGCCAGCTTTGATGAGCTTA...........................22129130001001000010110001000000001000140006003000000020010000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000
............................................................................ACAGCCAGCTTTGATGAGC..............................192701081921486698424514326927066327199446240460202745912301251138818303022077790118604474112132427436149046145610810451895189103434160181324917812117685269342412136765472622232015632037019187245314810001894521001230181212125111052000000030000001001000510010100100000000001110002000000000010100000000000
............................................................................ACAGCCAGCTTTGATGAG...............................182536322884210581031139953172661309033219826011107368298535598141313439810532941359067654138125079236865652110437124695540675202470454124110284101484901745694219021226130370350131911858216507007694110012411831111210003700100101000000000000601440010000000000000003030001000000000000000000000000
............................................................................ACAGCCAGCTTTGATGAGCTT............................2113514849719744386718233305310260561281527110801479551395964642116015412082049355052497139123628048463117243202041242416135624211301857432122010212717001063922001103405408114152206001100010030700071000000000100000000000020150000000000000000000000000000000000000000000000000000
............................................................................ACAGCCAGCTTTGATGAGCT.............................20284145352143442402322426353163245277211332178160014021892170414271061182908541297123344025667431165808786809679555109912241415831002122848010221358125236111311129011245237542702841125111155883896883709666103237641015388954540135061388901948822594989414340910005902040336812018648436792876141818854347412159451031011198021491515121120282400012701001100201133080520012435000000000004510101000020000010000000100000
.............................................................................CAGCCAGCTTTGATGA................................1622000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000
.............................................................................CAGCCAGCTTTGATGAGCTT............................2018303111070000000000200111005003000200010043000210000010010110100000000200071000000000000000000011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................................................................CAGCCAGCTTTGATGAGCTTA...........................2112000000000000000000000000000000000000000000000000000000000200000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
.............................................................................CAGCCAGCTTTGATGAG...............................1723000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000000000
.............................................................................CAGCCAGCTTTGATGAGC..............................182263217097313225213200280004010023411047306432410161008004000032021007213110030000010731461201130000380811000020421100100100000000400000010000000000000000000000000000000012000000009000000000000000
.............................................................................CAGCCAGCTTTGATGAGCT.............................19223965073233427032715272731725201481312033182284852552973271144941024977253711453401263613494123745852112402463894152643573014295311291276900763181128116100000203171111000000000100000200001121000020000000100000000000000000000000009000000000000000
..............................................................................AGCCAGCTTTGATGAGCT.............................1821506566423755281733582624411140113716862149401598018423688612222149171961445215171442844511134212102752892744531643061104414022320105135284625111696069191949921006841442452617112024631612914614513157313281110161262975015016746301101133100431400000201200002000010001110200000010000010000000000000000000000100000
..............................................................................AGCCAGCTTTGATGAGCTTA...........................201551030001010000010001001007002000000000350000270000000010100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
..............................................................................AGCCAGCTTTGATGAGCTT............................191127536195505811125205392374130352524633421311528071618317302311730302041203621101569022853201147017020000011000010103000000000001030000000000000040000000000000000000000000000000000000000010000000000000000000000000
...............................................................................GCCAGCTTTGATGAGCTT............................18110713584094573529419036410042143251121727103704015234252401900511016121523210115670401200304280001110000100001000000000000000004040001100000000050000000000003000020000000100000000000000000000000000000000000000000
...............................................................................GCCAGCTTTGATGAGCTTAG..........................2011000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
...............................................................................GCCAGCTTTGATGAGCTTA...........................19125015001000000000100000000000200000000003000000000000000000300000701000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
................................................................................CCAGCTTTGATGAGCTTA...........................1816000000000000000000000000000200000000022000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
................................................................................................TAGCGTAATGTATCGTACATCAA......2313000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000
................................................................................................TAGCGTAATGTATCGTACATC........2111000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
................................................................................................TAGCGTAATGTATCGTACATCA.......2211000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000
................................................................................................TAGCGTAATGTATCGTACA..........1911000000000000000000000000000000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
................................................................................................TAGCGTAATGTATCGTACATCAAATCAT.2811000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
Antisense strand
                                                                                                                             SizeBlastTotalV098 dcr-2[L811fsX] ovaryV064 ago2[414], ovary, AGO1IPSRR032095 AGO1 IP dcr2 knockdown V065 dcr-2[L811fsX], ovary, AGO1IPV082 embryo 14-24hrV063 CS,ovary,AGO1IPV018 Kc167 cellV088 r2d2[1] ovary total RNA  V097 CS ovaryV066 r2d2[1], ovary, AGO1IPV011 Sg4V076 ML-DmBG3-C2V021 ML-DmD21 cellV026 1182-4H cellV008 S2-DRSCV009 CMEL1V067 S2-NPGSM424740 S2 pKF63 stably transfected V081 embryo 2-6hrV020 S2R+ cellV033 CMEW1 Cl.8+ cellV089 ago2[414] ovary total RNA  GSM280088 S2cell (AGO1IP) V024 kc167 cellSRR014271 Embryo_0-2hrs rep1_w1118_0_2hr_Emb V025 S2R+ cellV001 IR-SRR029031 loqs-ORF knockdown V002 IR+V019 GM2 cellSRR014281 Ovary_rep1 wK(fem)_x_w1118(male)_F1 SRR014278 Ovary_rep1 w1118(fem)_x_Har(male)_F1 S33 KC-48 #2SRR014279 Ovary_rep1 w1118(fem)_x_wK(male)_F1 SRR014280 Ovary_rep1 w1118_P SRR014276 Ovary_rep1 NA(fem)_x_Har(male)_F1 V028 CMEW1 Cl.8+ cellSRR014268 Embryo_0-2hrs rep1_HAR_0_2hr_Emb SRR014269 Embryo_0-2hrs rep1_LK_0_2hr_Emb SRR014275 Ovary_rep1 LK_P SRR031696_2152 SRR014274 Ovary_rep1 LK(fem)_x_Har(male)_F1 GSM379052 Aub Heterozygote GSM424739 S2 parental GSM379063 Vasa Heterozygote SRR029028 untreated (mock) GSM379057 Krimp Mutant V010 MLDmD20c5V090 CS  male total RNA  SRR014277 Ovary_rep1 NA_P S32 s2+48 #2GSM379066 Zuc Mutant SRR014273 Ovary_rep1_Har_P GSM379054 Flam Heterozygote GSM379050 Armi Heterozygote GSM379064 Vasa Mutant GSM379065 Zuc Heterozygote SRR014270 Embryo_0-2hrs rep1_NA_0_2hr_Emb V083 male, one daySRR029032 r2d2 knockdown SRR001345 ago2 non-beta-eliminated GSM379051 Armi Mutant SRR014272 Embryo_0-2hrs rep1_wK_0_2hr_Emb SRR029030 dcr-2 knockdown SRR029033 lacZ knockdown SRR014282 Ovary_rep1 wK_P GSM379060 SpnE Heterozygote S29 male head #1V084 female, one dayGSM379058 SRR029029 dcr-1 knockdown GSM379056 Krimp Heterozygote S31 s2+48 #1S35 KC+48 #2SRR032094 ago2 knockdown GSM379053 Aub Mutant GSM379061 Squ Heterozygote GSM379062 Squ Mutant SRR031703 Total small RNAs from r2d2 homozygous flies S34 KC+48 #1OSS6 Drosophila OSS cell small RNA libraries, rep2 SRR029633 total small RNAs from hen1 homozygous flies OSS2 Drosophila OSS cell small RNA libraries, rep1 SRR031699 Total small RNAs from dcr-2 homozygous flies S30 s2-48 #2SRR031697 Total small RNAs from dcr-2 heterozygous flies V075 ML-DmBG1-C1SRR031701 Total small RNAs from r2d2 heterozygous flies OSS7 Drosophila OSS cell small RNA libraries, rep3 SRR031692 Total small RNAs from Oregon R GSM379067 SpnE Mutant GSM280084 loqs-/- ovaries (18-29nt) GSM379055 Flam Mutant S13 6-10h #2 (11)SRR032093 ago1 knockdown S12 6-10h #1 (10)S16 KC -48 #1OSS8 Drosophila OSS cell small RNA libraries, rep4 SRR001338 IR non-beta-eliminated GSM280085 WT testes (18-24nt) S22 female head #1SRR001344 dcr-2 beta-eliminated GSM424741 S2 pKF63 transiently transfected SRR001347 ago2 untreated GSM280082 WT ovaries (18-29nt) S28 0-2d pupaeGSM280083 dcr-2-/- ovaries (18-29nt) SRR001664 homozygous dcr-2_untreated SRR001341 WT_males non-beta-eliminated SRR001339 WT_females non-beta-eliminated GSM379059 S24 male body #2SRR001349 heterozygous dcr-2_untreated S8 S2-48,+48, KC-48, +48 mixS10 2-6h #1 (8)SRR001343 dcr-2 non-beta-eliminated GSM343287 Drosophila Toll 10b mutant embryos S9 0-1h #3 (7)S27 0-1d Pupae (w)S15 S2 -48 #1S1 male headSRR032096 AGO2 IP dcr2 knockdownS2 female headSRR031704 2-O-methylated small RNAs from r2d2 homozygous flies SRR010954 Aub trans-heterozygotes, oxidized S14 0-1hr #1 (A)S7 12-24hr embryoGSM239052 S2 cells, non beta-eliminated S23 female body #2S11 2-6h #2 (9)S21 disk #2SRR010953 Aub heterozygotes, oxidized S19 2-4day pupae #1S5 imaginal discGSM280087 S2cell (AGO2IP) GSM467730 r2d2 SRR032092 mock oxidized GSM467729 WT SRR010959 Ago3 IP in heterozygotes S26 1st instar #2GSM180328 adult heads (female heads, male heads) SRR001348 ago2 oxidized SRR031700 2-O-methylated small RNAs from dcr-2 homozygous flies S25 1st instar #1SRR031698 2-O-methylated small RNAs from dcr-2 heterozygous flies SRR001346 ago2 beta-eliminated SRR001337 WT_females beta-eliminated S17 3rd inster #1GSM180333 late embryo (12-24) S4 female bodyS3 male bodyGSM180332 mid embryo (6-10) GSM239041 fly heads, non beta-eliminated GSM467731 loq S20 2-4day pupae#2GSM180337 tissue culture cells (S2 only) GSM180331 early embryo (2-6) S18 3rd inster #2SRR010955 Aub IP in Ago3 heterozygotes SRR023400 total RNA extracted from P19 cells SRR010956 Piwi IP in Ago3 heterozygotes GSM180335 imaginal discs SRR031702 2-O-methylated small RNAs from r2d2 heterozygous flies SRR031693_4 SRR010958 Piwi IP in Ago3 trans-heterozygotes GSM180329 adult bodies (female bodies, male bodies) GSM180330 very early embryo (0-1) GSM180336 pupae: 0-1 day, 0-2 day, 2-4 day GSM180334 larvae: 1st instar and 3rd instars SRR023399 RNA bound by P19 protein SRR001340 IR beta-eliminated SRR010960 wt, oxidized GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006) GSM246084 D. melanogaster adult male heads 454 GSM239051 S2 cells, beta-eliminated SRR001342 WT_males beta-eliminated SRR010952 Ago3 trans-heterozygotes, oxidized SRR023197 RNA Library from S2 control cells GSM154618 23-29 nucleotide RNAs from Drosophila melanogaster ovaries SRR010957 Aub IP in Ago3 trans-heterozygotes SRR023407 RNA bound by NLS-P19 protein SRR010951 Ago3 heterozygotes, oxidized GSM239050 fly heads, beta-eliminated GSM280086 WT ovaries (AGO2IP) GSM154621 piRNAs associated with Ago3 from Drosophila melanogaster ovaries GSM154620 piRNAs associated with Piwi from Drosophila melanogaster ovaries SRR023402 total RNA extracted from NLS-P19 cells GSM154622 piRNAs associated with Aubergine from Drosophila melanogaster ovaries GSM266765 Siomi Lab fruit fly S2 AGO2 Cloned Sequences v1.0