logoC.briggsae small RNAs


Summary

InteRNA Genomics small RNA analysis pipeline miR-Intess™. Feb 16, 2009.


Total number of analysed libraries: 1

Total number of raw reads: 65,889

Total number of accepted reads: 61,812

Total number of mapped reads: 41,877

Total number of cloned miRBase miRNAs: 87

Total number of homolog miRBase miRNAs: 28

Total number of novel identified miRNAs: 15

Parameters of analysis
Reference organism: cbriggsaeAssembly: CB3 
Ensembl database: UCSC database: cb3Mirbase version: 12.0
Min read length: 18Max read length: 30Min mapped read length: 18
READ trimming: THREE_PRIME_A_MISMATCH_AND_ALL_TRIMEditing analysis: 0 
Hairpin classification:
Distance from stem base: 5Distance from loop: 0Max distance between mature and star: 50
Min mature length: 20Max mature length: 24Relative read abundance for signature calculation:0.4
Max mature GC: 0.9Min Dicer/Drosha overhang: 0Max Dicer/Drosha overhang: 3
Min required reads for novel confident miRNA: 5Min required libs for novel confident miRNA: 1Lib/read overlap for confident novel miRNAs: AND
Randfold threshold for confident novel miRNAs: 0.01Species for conservation analysis: celegans cbrenneri cremanei cjaponica ppacificus RNA forester threshold for homology: 0.3

©2008 InteRNA Genomics B.V.