logoC.elegans small RNAs


Summary

InteRNA Genomics small RNA analysis pipeline miR-Intess™. Feb 16, 2009.


Total number of analysed libraries: 1

Total number of raw reads: 184,340

Total number of accepted reads: 181,291

Total number of mapped reads: 160,272

Total number of cloned miRBase miRNAs: 112

Total number of homolog miRBase miRNAs: 0

Total number of novel identified miRNAs: 1

Parameters of analysis
Reference organism: celegansAssembly: ce4 
Ensembl database: caenorhabditis_elegans_core_52_190UCSC database: ce6Mirbase version: 12.0
Min read length: 18Max read length: 30Min mapped read length: 18
READ trimming: THREE_PRIME_A_MISMATCH_AND_ALL_TRIMEditing analysis: 0 
Hairpin classification:
Distance from stem base: 5Distance from loop: 0Max distance between mature and star: 50
Min mature length: 20Max mature length: 24Relative read abundance for signature calculation:0.3
Max mature GC: 0.9Min Dicer/Drosha overhang: 1Max Dicer/Drosha overhang: 3
Min required reads for novel confident miRNA: 5Min required libs for novel confident miRNA: 1Lib/read overlap for confident novel miRNAs: AND
Randfold threshold for confident novel miRNAs: 0.01Species for conservation analysis: cbrenneri cbriggsae cremanei cjaponica ppacificus RNA forester threshold for homology: 0.3

©2008 InteRNA Genomics B.V.