logoC.elegans small RNAs


miR expression

cel-mir-1019

cel-mir-1019

sblock9 (miRBase cel-mir-1019) [miRNAknown_lenNOK_multiarm_DicerNOK_shortStem_mirtron]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
miRBase cel-mir-10190.029nono0.36/0.4224/25/0.50nd
nd
nd
nd
0
0
0
0
0
0
0
0
0
0
3
7
5arm
3arm
1
1
1
0
0.20
0.35
2
5
0030
sblock9 hairpin
  readsmiRBase family seed
seed     --------------UGAGCAU---------------------------------------------------------------------------------------------------     0novel
seed     --------------------------------------------------------------------------------UGUAAU-----U----------------------------     0novel
  lencloning frequencies
   
cel-miR-1019     -------------------------------------------------------------------------------CTGTAAT-----TCCACATTG----------CTTTCCAG--     24
cel-miR-1019*     -------------GTGAGCATTGTT---------------------CGAGTTTCATTTT-------------------------------------------------------------     25
celegans     -------------GTGAGCATTGTT---------------------CGAGTTTCATTTTTAATAAAATTTATTTAAAAACTGTAAT-----TCCACATTG----------CTTTCCAG--      
cremanei     -------------GTGAGCATTGTA---------------------TGAGCCA-----------------ATCTGAAAAAACCTAC-----TTCAGGTTGAGAATACAGTTTTAA-----      
cjaponica     -------------GTGAGCATAGTT---------------------CGAGTTACAGTA------------ATCCAATGACTACTGT--ATCTCCACTTTG----------CTTTC-----      
cbrenneri     -------------GTGAGCATCGTT---------------------GGATTTATAGTTTTTTT-------TTCAAAAAATTCTAACTATAATCCACTTTG----------CTTAA-----      
ppacificus     TTGTTGAAGAACTGTGAGCATTATTGATCTTTGATCTTTTGAATTGTGAATCATATTTCGGAC-------ACCTGATCAGTCTAAT--TAGTGCTTTTGG----------TTTACAATGA      
                   ********  *                       **                          *  *            * *   * *           **             
 >>>>>             ++++++++++++                     ++++++++++++++++++++++++++++++++++++++++     +++++++++          ++++++++  >>>>>M04C9.5 M04C9.5 dyf-5
celegans                  ..(((((.(((.                     .(((((.(((((((((..........))))))).)).)))     )).))).))          ))).....       1.000 -13.10
cremanei                  .((((.((((((                     (....((                 (((((((.........     )))))))))...))))))))))).          1.000 -14.80
cjaponica                  ..(((((.(((.                     .(((.(((((((            .((....)).))))))  ).))).))).))          )))..          1.000 -19.40
cbrenneri                  .((((((..((.                     (((.((((((((..(((       (....))))....)))))))))))))..))          )))).          1.000 -17.10
ppacificus     ((((((..((((((.((((((((.......((((((.((((((.((((...)))).)))))).       ....))))))......  )))))))).)))          ))).))))))     0.996 -23.82

celeganschromosome:I:9361588:9361656:1Same_strand|Intronic_coding|M04C9.5|M04C9.5 ## Same_strand|Boundary_coding|M04C9.5|M04C9.5 ## M04C9.5|protein_coding|dyf-5|dyf-5 encodes a putative MAP kinase orthologous to human MAK/ICK (OMIM:154235), Chlamydomonas reinhardtii LF4, and Leishmania mexicana MPK9. DYF-5 negatively regulates cilial length, restricts KAP-1 to middle ciliary segments, is required for normal localization of six IFT components, and is required for OSM-3 to comigrate normally with IFT particles. DYF-5 is also required for dye-filling of amphid and phasmid neurons and for normal chemotaxis, dauer formation, and male mating. DYF-5 is expressed in head neurons (including amphid neurons), tail neurons (including phasmid neurons), CAN cells, excretory canal neurons, posterior lateral ganglion neurons and in many male tail cells. dyf-5 mutant cilia are abnormally elongated, either failing to enter the amphid channel or accumulating IFT proteins at their distal ends, whereas DYF-5 overexpression results in truncated cilia. the dyf-5 promoter region contains an X-box, predicted to be bound and transcriptionally activated by DAF-19, and dyf-5 is regulated by DAF-19 in vivo. dyf-5 animals are slightly shorter than normal. [Source: WormBase] ## {SimpF: WRM0625aB10 1 fosmid,WRM0632bF02 1 fosmid,WRM0612aC09 1 fosmid,WRM064dG06 1 fosmid} ## {MIR: cel-mir-1019}
cremaneichromosome:chrUn:17538323:17538431:1Opposite_strand|Intronic_coding|NM_059002
cjaponicachromosome:chrUn:95002809:95002917:-1Opposite_strand|Intronic_coding|NM_007674 ## Same_strand|Boundary_coding|NM_060092 ## Same_strand|Boundary_non-coding|NM_199946
cbrennerichromosome:chrUn:140316700:140316808:-1Same_strand|Boundary_coding|NM_060092 ## Same_strand|Boundary_non-coding|NM_199946
ppacificuschromosome:chrUn:64451867:64451967:1Opposite_strand|Intronic_coding|NM_132581 ## Same_strand|Intronic_coding|NM_203770


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