logoC.elegans small RNAs


miR expression

cel-mir-1829a

cel-mir-1829a

Cloning frequencies
absoluteCE1
cel-mir-1829a 5arm3
cel-mir-1829a 3arm4
normalizedCE1
cel-mir-1829a 5arm0.002
cel-mir-1829a 3arm0.003
cel-mir-1829a relative cloning frequencies

sblock68 (miRBase cel-mir-1829a) [miRNAknown_lenNOK_cloningOK_multiarm_DicerNOK_shortStem]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
miRBase cel-mir-1829a0.011nono0.32/0.3819/22/0.500.0
0.0
0.3
0.0
3
4
0
0
1
1
0
0
0
3
2
3
5arm
3arm
1
1
nd
nd
0.14
0.16
1
1
711-1
sblock68 hairpin
  readsmiRBase family seed
seed     -------------------------------------------AACCAUU-------------------     4novel
seed     -A----GGGGAC---------------------------------------------------------     3novel
  lencloning frequencies
   CE1
      ------------------------------------------CAACCATTGGAATTTCTCT--------     194
      AA----GGGGACTTCTAATTGTTTG--------------------------------------------     212
      AA----GGGGACTTCTAATTGTTTGT-------------------------------------------     221
cel-miR-1829a     ------------------------------------------CAACCATTGGAATTTCTCTATT-----     22-
celegans     AA----GGGGACTTCTAATTGTTTGTAAAAAATCGAGTATTACAACCATTGGAATTTCTCTATT-----      
cjaponica     AAAATCGGGGAA----AAATGTTTGAAAAAAAT---ATATTTCAACCAT-----TTTTTACACGACCAC      
      **    *****     ** ****** *******    **** *******     *** *  *             
 +++++++    ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++     +++++K09A9.5 K09A9.5 gas-1
celegans     .(    (((((.(((((((.(((.((((...........))))))).))))))).))))))...          1.000 -14.10
cjaponica     ....(((..(((    (((((.((((((.....   ...)))))).)))     )))))...)))....     1.000 -13.20

celeganschromosome:X:15588233:15588292:-1Same_strand|Intronic_coding|K09A9.5|K09A9.5 ## K09A9.5|protein_coding|gas-1|The gas-1 gene encodes a 49 kDa subunit of mitochondrial complex I that is required for oxidative phosphorylation, resistance to volatile anesthetics, fecundity, and normal lifespan. gas-1 is expressed in the nematode neuromuscular system. its subcellular distribution appears to be mitochondrial. drug effects on mutants versus wild-type indicate that gas-1 functions at least partially through presynaptic effects. gas-1 mutants have strongly reduced Complex I-dependent metabolism in mitochondria, while Complex II-dependent metabolism is conversely increased in mutants. gas-1 mitochondria look structurally normal. [Source: WormBase] ## {SimpF: WRM061cB03 -1 fosmid,WRM0611bC07 -1 fosmid,WRM0628aD06 -1 fosmid,WRM0614bD09 -1 fosmid} ## {MIR: cel-mir-1829a}
cjaponicachromosome:chrUn:89502738:89502837:1Same_strand|Intronic_coding|NM_005587


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