miR-Intess: cel-mir-77

cel-mir-77

Cloning frequencies
absolute	CE1
cel-mir-77 5arm	35
cel-mir-77 3arm	14357
normalized	CE1
cel-mir-77 5arm	0.024
cel-mir-77 3arm	10.029

sblock26 (miRBase cel-mir-77) [miRNAknown_cloningHIGH_multiarm_DicerOK_randfoldOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
miRBase cel-mir-77	0.003	no	no	0.44/0.58	18/25/0.99	0.0 0.0	1.5 0.0	29 13758	0 0	1 1	0 0	23 18	5 6	5arm 3arm	1 1	nd nd	0.17 0.14	2 2	14392	1	2	1

		reads	miRBase family seed
seed	----------------------------------------------------------------------UCAUCAG-------------------------------------------	14081	miR-77
seed	-----------------------------------------------------------------------CAUCAGG------------------------------------------	142	novel
seed	--------------------------------------------------------------------------CAGGCCA---------------------------------------	130	novel
seed	------------------------AUGGUUG-----------------------------------------------------------------------------------------	35	novel
seed	---------------------------------------------------------------------UUCAUCA--------------------------------------------	2	novel
seed	------------------------------------------------------------------------AUCAGGC-----------------------------------------	2	novel
		len	cloning frequencies
			CE1
	---------------------------------------------------------------------TTCATCAGGCCATAGCTGTCC------------------------------	21	13758
	-------------------------------------------------------------------------TCAGGCCATAGCTGTCCAAA---------------------------	20	128
	----------------------------------------------------------------------TCATCAGGCCATAGCTGTCCA-----------------------------	21	120
cel-miR-77	---------------------------------------------------------------------TTCATCAGGCCATAGCTGTCCA-----------------------------	22	85
	---------------------------------------------------------------------TTCATCAGGCCATAGCTG---------------------------------	18	72
	---------------------------------------------------------------------TTCATCAGGCCATAGCTGTC-------------------------------	20	65
	---------------------------------------------------------------------TTCATCAGGCCATAGCTGTCCAA----------------------------	23	58
	---------------------------------------------------------------------TTCATCAGGCCATAGCTGTCCAAA---------------------------	24	22
	----------------------------------------------------------------------TCATCAGGCCATAGCTGTCC------------------------------	20	21
	-----------------------GATGGTTGTGCTCTGAGGAAAT---------------------------------------------------------------------------	22	18
	-----------------------GATGGTTGTGCTCTGAGG-------------------------------------------------------------------------------	18	11
	---------------------------------------------------------------------TTCATCAGGCCATAGCTGT--------------------------------	19	11
	---------------------------------------------------------------------TTCATCAGGCCATAGCTGTCCAAAT--------------------------	25	10
	-----------------------GATGGTTGTGCTCTGAGGAA-----------------------------------------------------------------------------	20	5
	--------------------------------------------------------------------TTTCATCAGGCCATAGCTGTCC------------------------------	22	2
	-----------------------------------------------------------------------CATCAGGCCATAGCTGTCC------------------------------	19	2
	-----------------------GATGGTTGTGCTCTGAGGAAA----------------------------------------------------------------------------	21	1
	----------------------------------------------------------------------TCATCAGGCCATAGCTGTC-------------------------------	19	1
	-------------------------------------------------------------------------TCAGGCCATAGCTGTCCAA----------------------------	19	1
	-------------------------------------------------------------------------TCAGGCCATAGCTGTCCA-----------------------------	18	1
celegans	GCATCTGCCAAACCGCCCGTTTGGATGGTTGTGCTCTGAGGAAATA--------CGCACAGAATGTCATTTCATCAGGCCATAGCTGTCCAAATTGGTATAGAGTTTG------------
cbriggsae	---------GAGCTGTGAATTTGGATAGCTGTGCTCTGAGGAAATAACAATAGTCACACCCCATGTCATTTCATCAGGCCATAGCTGTCCAAATTT--CTCG-GTTC-------------
cjaponica	-------GCGCATTTTCAGTTTGGATGACTGTGCTCTGAAGAAATACTGATGATCGTCC---GTAGCATTTCATCAGGCCATAGTTGTCCAAACTG--AGAG-TTGTGTGTTTTTTCACT
cbrenneri	---------GAGCCAATAATTTGGATGGCTGTGCCTTGAAGAAATACAGTTA----------GTGTCATTTCATCAGGCCATAGCTGTCCAAATTG--TTAG-GTTCGCCAGTTTTTTAG
cremanei	AACTGTAAAGAGCTGTTAGTTTGGATGGCTGTGCTCTGAGGAAATACGG-----CTCATAAAATGTCATTTCATCAGGCCATAGCTGTCCAAATTA--TCAATGTTCGAAAGTT------
	***** * * ****** * **************** ****** * *
celegans	........(((((((((.(((((((((((((((..((((.(((((. .(((.....))).))))).))))..))))))))))))))).)))...).)))))	1.000 -35.40
cbriggsae	((((((.(((((((((((((((((..((((.(((((.(((..............))).))))).))))..))))))))))))))))) ..)) ))))	0.998 -41.64
cjaponica	((((((((((((((((((..((((((..((((.(((((.((.(((......) )))).))))).))))..))))))..))))))))) )))) ).))))...........	0.959 -47.20
cbrenneri	((((((((((((((((((((((((.(((((.((((((((.... .))).))))).))))).))))))))))))))))) )).) )))).............	0.995 -44.30
cremanei	((((.....((((((.(((((((((((((((((..((((.(((((..(( (.........)))))))).))))..))))))))))))))))) .))..))))...))))	1.000 -39.90

celegans	chromosome:II:12519183:12519282:1	intergenic ## {SimpF: WRM0641dA02 1 fosmid,WRM063bE08 1 fosmid,WRM0630aA08 1 fosmid,WRM061aD04 1 fosmid,WRM0636cG04 1 fosmid,WRM064cG11 1 fosmid,WRM0629aH07 1 fosmid,WRM0641bG10 1 fosmid} ## {MIR: cel-mir-77}
cbriggsae	chromosome:chrII:12520819:12520913:-1	Opposite_strand\|Intronic_coding\|NM_077872 ## {MIR: cbr-mir-77-1}
cjaponica	chromosome:chrUn:40079835:40079941:1	Opposite_strand\|Intronic_coding\|NM_001101877
cbrenneri	chromosome:chrUn:11565280:11565377:-1	Opposite_strand\|Intronic_coding\|NM_031741
cremanei	chromosome:chrUn:53585347:53585453:-1	intergenic

miR-Intess™

cel-mir-77

sblock26 (miRBase cel-mir-77) [miRNAknown_cloningHIGH_multiarm_DicerOK_randfoldOK]