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Comment: Based on this graph the experimental cut-offs for the "CloningOK" parameter of the hairpins are defined. This parameter is used for distinguishing confident novel miRNA from other hairpins. In this graph the number of loci passing various reads/libs thresholds are plotted, and fraction of miRNA loci vs other loci are calculated for different combinations of threshold reads/libs and thresholds are selected such that the maximal number of known miRNA loci were above the treshold while keeping the number of other loci to the minimum. |
See underlying thresholds distribution table for more details. |