miR-Intess: cel-mir-86

cel-mir-86

Cloning frequencies
absolute	CE1
cel-mir-86 5arm	3802
normalized	CE1
cel-mir-86 5arm	2.656

block3302 (miRBase cel-mir-86) [miRNAknown_cloningHIGH_randfoldOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
miRBase cel-mir-86	0.001	no	no	0.40/0.47	19/25/0.97	0.0	0.0	3157	0	1	0	15	6	5arm	1	nd	0.22	2	3802	1	na	na

Families
Member of family miR-785/86 (seed AAGUGAA): cel-mir-86, cel-mir-785

		reads	miRBase family seed
seed	------------------------------------AAGUGAA----------------------------------------------------------------------------------------------------	3759	miR-785/86
seed	----------------------------------------GAAUGCU------------------------------------------------------------------------------------------------	25	novel
seed	-------------------------------------AGUGAAU---------------------------------------------------------------------------------------------------	18	novel
		len	cloning frequencies
			CE1
cel-miR-86	-----------------------------------TAAGTGAATGCTTTGCCACAGTC-------------------------------------------------------------------------------------	23	3157
	-----------------------------------TAAGTGAATGCTTTGCCACAGTCT------------------------------------------------------------------------------------	24	306
	-----------------------------------TAAGTGAATGCTTTGCCACAGT--------------------------------------------------------------------------------------	22	168
	-----------------------------------TAAGTGAATGCTTTGCCAC-----------------------------------------------------------------------------------------	19	90
	---------------------------------------TGAATGCTTTGCCACAGTC-------------------------------------------------------------------------------------	19	22
	-----------------------------------TAAGTGAATGCTTTGCCACAGTCTT-----------------------------------------------------------------------------------	25	20
	------------------------------------AAGTGAATGCTTTGCCACAGTC-------------------------------------------------------------------------------------	22	17
	-----------------------------------TAAGTGAATGCTTTGCCACAG---------------------------------------------------------------------------------------	21	14
	-----------------------------------TAAGTGAATGCTTTGCCACA----------------------------------------------------------------------------------------	20	4
	---------------------------------------TGAATGCTTTGCCACAGTCT------------------------------------------------------------------------------------	20	3
	------------------------------------AAGTGAATGCTTTGCCACAGTCT------------------------------------------------------------------------------------	23	1
celegans	--------------------CGTGTCCACGCCGTCTAAGTGAATGCTTTGCCACAGTCTT-CGATGTT-CTGAAA--TGAAGCCTGGGCTCAGATTCGCTT-AGGCCGGAGTTTGACACGGCA--------------------
cbriggsae	-CCCTTCCTCTTCAACAAACCGAGTCAATGCCGTTTAAGTGAATGCTTTGCCACAGTCTT-CGATCAT-GTCACA--TGAAGCCTGGGCTTAGATTCACTT-AGGCCGGCGTTCCTTGACTCAGCCTTTTTTTCTTCTTGGG-
cjaponica	-----------------------------------TAAGTGAATGCTTTGCCACAGTCTTCCGATCTCTGTCGAATGTGAAGCCTGGGTCGAGATTCGCTT-A----------------------------------------
cbrenneri	-CCCCTTTTTAAAAATACAACGAGTCGGCGCCGTCTAAGTGAATGCTTTGCCACAGTCTT-CGACCAT-GTCAAA--GGAAGCCTGGGCTCAGATTCGCTT-AGGCCGGCGTTCGACACGTTTCTTTGTGGGCTGCTTTGGGG
ppacificus	-----CATGCCTCGATATTAGAATTCGGTCCCAAGCAAGTGAATGTCCGCACAGCGCATC-CACTAGTAGTCACA---GTGGCATCGCCGTCCATTCCTTTCACTCCTCCGTTTC----------------------------
cremanei	TCCCATACTTTTCAA----ACGAGTCGGCGCCGTCTAAGTGAATGCTTTGCCACAGTCTT-CGACCAT-GTCAAA--TGAAGCCTGGGCTAAGATTCGCTT-AGGCCGGCGTTTTTCGACACGGATCTTCAACTTTGGTGA--
	******* * * * * * * ** * * ** *
	+++++ ++++++++++++++++++++++++++++++++++++++++ +++++++ ++++++ ++++++++++++++++++++++++ +++++++++++++++++++++ +++++	Y56A3A.7b Y56A3A.7 Y56A3A.7
	+++++ ++++++++++++++++++++++++++++++++++++++++ +++++++ ++++++ ++++++++++++++++++++++++ +++++++++++++++++++++ +++++	Y56A3A.7a Y56A3A.7 Y56A3A.7
celegans	((((((.((.((((((((((((((.((..(((.(((.((( ((.(... ....). ))))).))))))..)))))))))) ))).))).))..))))))...	1.000 -42.60
cbriggsae	(((.................(((((((((((((((((((((((.((..(((.(((.((( ((..... ...... ))))).))))))..)))))))))) )))).)))))....)))))).................)))	0.842 -45.18
cjaponica	(((((((((.(((.(((.(((.((((((.((......)).)).)))).)))))).))))))))))) )	1.000 -24.50
cbrenneri	((((..........(((((((.(((((((((((((((((((((.((..(((.(((.((( ((((... )))... .)))).))))))..)))))))))) )))).))))).)))).))).....)))).........))))	0.824 -55.61
ppacificus	...............(((..(((........(((.(((((..((....(((.((( ((((.((....))) )))).)))))))..))))))))........))).)))	0.903 -21.99
cremanei	(((((.......... .((.(((((((((((((((((((((.(((.(((.(((.((( ((((... )))... .)))).)))))).))))))))))) )))).)))))....)))).))............))).))	0.908 -49.00

celegans	chromosome:III:11936518:11936615:-1	Same_strand\|Intronic_coding\|Y56A3A.7a\|Y56A3A.7 ## Y56A3A.7\|protein_coding\|Y56A3A.7\|Y56A3A.7 [Source:RefSeq_peptide;Acc:NP_499557] ## {SimpF: CEOP3841 1 Operon,WRM0624cH06 -1 fosmid} ## {MIR: cel-mir-86}
cbriggsae	chromosome:chrIII:4132883:4133018:-1	Same_strand\|Intronic_coding\|NM_177732 ## Opposite_strand\|Intronic_coding\|NM_001043354 ## {MIR: cbr-mir-86}
cjaponica	chromosome:chrUn:58779718:58779855:1	intergenic
cbrenneri	chromosome:chrUn:102433287:102433423:1	Same_strand\|Intronic_coding\|NM_211666
ppacificus	chromosome:chrUn:50014221:50014326:1	Opposite_strand\|Intronic_coding\|NM_001086814
cremanei	chromosome:chrUn:4696101:4696232:1	intergenic

miR-Intess™

cel-mir-86

block3302 (miRBase cel-mir-86) [miRNAknown_cloningHIGH_randfoldOK]