logoC.elegans small RNAs


miR expression

cel-mir-87

cel-mir-87

Cloning frequencies
absoluteCE1
cel-mir-87 5arm1
cel-mir-87 3arm171
normalizedCE1
cel-mir-87 5arm0.001
cel-mir-87 3arm0.119
cel-mir-87 relative cloning frequencies

sblock57 (miRBase cel-mir-87) [miRNAknown_cloningHIGH_multiarm_randfoldOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
miRBase cel-mir-870.001nono0.45/0.5518/22/0.980.0
0.0
0.0
0.0
1
145
0
0
1
1
0
0
40
12
-15
9
5arm_loop_3arm
3arm
1
1
nd
nd
0.40
0.09
6
1
1721nana
Families
Member of family miR-233/356/87 (seed UGAGCAA): cel-mir-233, cel-mir-87
sblock57 hairpin
  readsmiRBase family seed
seed     -----------------------------------------------------------------------------------UGAGCAA------------------------------------------     166miR-233/356/87
seed     ------------------------------------------------------------------------------------GAGCAAA-----------------------------------------     3novel
seed     ---------------------------------------------------------------------------------------CAAAGUU--------------------------------------     2novel
seed     -------------------------------------------------------------GCUG--UCA--------------------------------------------------------------     1novel
  lencloning frequencies
   CE1
cel-miR-87     ----------------------------------------------------------------------------------GTGAGCAAAGTTTCAGGTGTGC----------------------------     22145
      ----------------------------------------------------------------------------------GTGAGCAAAGTTTCAGGTGTG-----------------------------     2118
      -----------------------------------------------------------------------------------TGAGCAAAGTTTCAGGTGTGC----------------------------     213
      ----------------------------------------------------------------------------------GTGAGCAAAGTTTCAGGTGT------------------------------     202
      --------------------------------------------------------------------------------------GCAAAGTTTCAGGTGTGC----------------------------     182
      ----------------------------------------------------------------------------------GTGAGCAAAGTTTCAGGTG-------------------------------     191
      ------------------------------------------------------------CGCTG--TCAGATTGGTCGTAG--------------------------------------------------     201
celegans     -------------------GGTTGTG-CCATCCGGCCGCCTGATACTTTCGTCTCAACCTCGCTG--TCAGATTGGTCGTAGGTGAGCAAAGTTTCAGGTGTGCCGGAACACACCC----------------      
cbriggsae     -------------------GGTTGTGCCCACCCGGCCGCCTGATACTTTCGTCTCAACCTCGCTG--TCAGA-ATGTCGTAGGTGAGCAAAGTTTCAGGTGTGCCGGAACACACCC----------------      
cjaponica     ----------------------------------GCCGCCTGACACTTTCGTCTCAACCTCGCT---TCAGA-TGGCCGTAGGTGAGCAAAGTTTCAGGTGTGC----------------------------      
cbrenneri     -----------------------GTG-CCTTCCGGCCGCCTGATACTTTCGTCTCAACCTCGCTTC-TCTCA-TTGTCGTAGGTGAGCAAAGTTTCAGGTGTGCCGGAACACAC------------------      
ppacificus     -----------------GGGGTGACGTCATGCTCGTCGCCTGA-ACTTGTA-CTCAACCTCGTCA--ATAGA-TGAGCGCAGGTGAGCAAAGTTTCAGGTGTGCGAGCTAGCGTCCCCC-------------      
cremanei     AAACTTCTTCATCCATACAGGTTGTG-CCTTCCGGCCGCCTGACACTTTCGTCTCAACCTCGCTGTCTGATA-TAGTCGTAGGTGAGCAAAGTTTCAGGTGTGCCGGAACACATCCTCACTTTGCCCAGTTT      
                                        * ******* ****    **********         *     ** ************************                                  
 +++++                   +++++++ ++++++++++++++++++++++++++++++++++++++  +++++++++++++++++++++++++++++++++++++++++++++++++                +++++F10C2.2 F10C2.2 kup-1
celegans                        (((.((( ...(((((((((((((.(((((.(.((((.((((((((  ......))).)).))))))))))))).))))))).)))))).)))))).                     1.000 -44.60
cbriggsae                        ((.((((.....((((((((((((.(((((...(((.((((((.((  (.... ))).)).))))))).))))).))))))).)))))..)))))).                     0.994 -38.10
cjaponica                                       (((((((((.(((((...(((.((((((((   ..... .)).)).))))))).))))).))))))).))                                 1.000 -26.20
cbrenneri                            ((( ..((((((((((((((.(((((...(((.((((((.... ..... ....)).))))))).))))).))))))).))))))))))..                       0.999 -38.02
ppacificus                      ((((.(((((...((((((((((((( ((((... (((.(((((((((  ..... ))).)).)))))))..))))).)))))).))))))..)))))))))                  1.000 -50.30
cremanei     ..................(((.(((( ..((((((((((((((.(((((...(((.((((((((((.....) ))).)).))))))).))))).))))))).))))))))))).)))...............     0.918 -47.30

celeganschromosome:V:12038665:12038758:-1Same_strand|Intronic_coding|F10C2.2|F10C2.2 ## F10C2.2|protein_coding|kup-1|kup-1 encodes a novel protein that is conserved in C. briggsae, but contains no other known homologs. kup-1 is the upstream gene in an operon with pkc-1, which encodes two protein kinase C isoforms of the nPKC isotype. the polycistronic kup-1/pkc-1 mRNA is detected at low levels in embryos and larvae, but its expression greatly increases in adults. as loss of kup-1 activity via large-scale RNAi screens does not result in any obvious abnormalities, the precise role of kup-1 in C. elegans development and/or behavior is not yet known. [Source: WormBase] ## {SimpF: CEOP5312 1 Operon,WRM0626bF07 -1 fosmid,WRM063aG10 -1 fosmid,WRM0621bH11 -1 fosmid,WRM0622bH03 -1 fosmid} ## {MIR: cel-mir-87}
cbriggsaechromosome:chrV:6389696:6389789:1Same_strand|Intronic_coding|NM_031366 ## Opposite_strand|Intronic_coding|NM_001087279 ## {MIR: cbr-mir-87}
cjaponicachromosome:chrUn:17231967:17232100:1intergenic
cbrennerichromosome:chrUn:83992867:83992954:1Same_strand|Intronic_coding|NM_073615
ppacificuschromosome:chrUn:113454284:113454380:-1Same_strand|Intronic_coding|NM_001014737
cremaneichromosome:chrUn:126942557:126942686:-1Same_strand|Intronic_coding|NM_073615


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