miR-Intess: cel-mir-81

cel-mir-81

Cloning frequencies
absolute	CE1
cel-mir-81 3arm	9157.500
normalized	CE1
cel-mir-81 3arm	6.397

block8205 (miRBase cel-mir-81) [miRNAknown_cloningHIGH_randfoldOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
miRBase cel-mir-81	0.001	no	no	0.37/0.45	18/23/0.97	0.0	0.0	8025	0	1	0	20	8	3arm	1	nd	0.18	2	9184	1	na	na

Families
Member of family miR-80/81/82 (seed GAGAUCA): cel-mir-82, cel-mir-81, cel-mir-80, block4332_cand

		reads	miRBase family seed
seed	----------------------------------------------------------------------------------GAGAUCA-----------------------------------------------------	9046	miR-80/81/82
seed	-----------------------------------------------------------------------------------AGAUCAU----------------------------------------------------	66	novel
seed	--------------------------------------------------------------------------------------UCAUCGU-------------------------------------------------	63	novel
seed	-------------------------------------------------------------------------------------AUCAUCG--------------------------------------------------	8	miR-392
seed	------------------------------------------------------------------------------------GAUCAUC---------------------------------------------------	1	novel
		len	cloning frequencies
			CE1
cel-miR-81	---------------------------------------------------------------------------------TGAGATCATCGTGAAAGCTAGT---------------------------------------	22	8025
	---------------------------------------------------------------------------------TGAGATCATCGTGAAAGCTAG----------------------------------------	21	437
	---------------------------------------------------------------------------------TGAGATCATCGTGAAAGCTAGTT--------------------------------------	23	339
	---------------------------------------------------------------------------------TGAGATCATCGTGAAAGCT------------------------------------------	19	178
	----------------------------------------------------------------------------------GAGATCATCGTGAAAGCTAGT---------------------------------------	21	61
	-------------------------------------------------------------------------------------ATCATCGTGAAAGCTAGT---------------------------------------	18	60
	---------------------------------------------------------------------------------TGAGATCATCGTGAAAGC-------------------------------------------	18	53
	---------------------------------------------------------------------------------TGAGATCATCGTGAAAGCTA-----------------------------------------	20	14
	------------------------------------------------------------------------------------GATCATCGTGAAAGCTAGT---------------------------------------	19	8
	-------------------------------------------------------------------------------------ATCATCGTGAAAGCTAGTT--------------------------------------	19	3
	----------------------------------------------------------------------------------GAGATCATCGTGAAAGCTAG----------------------------------------	20	3
	----------------------------------------------------------------------------------GAGATCATCGTGAAAGCT------------------------------------------	18	2
	-----------------------------------------------------------------------------------AGATCATCGTGAAAGCTAGT---------------------------------------	20	1
celegans	--------------ATCAGTGCCATCGTGCCCAACAGTCGGTTTTCACCGTGATCTGAGAGCAAT-CCAAAAATGCTTTTCTGAGATCATCGTGAAAGCTAGTTGTTGGTCTACGGGCTTTTG-------------------
cbriggsae	------------------------------------GTCGGTTTTCACCTTGATCTGAGAGCAATAACCAGAATGCTTATCTGAGATCATCGTGAAAGCTAGT---------------------------------------
cjaponica	------------------------------------GTCGGTTTTCCCATTGATCTGAGAGCTAA---CTGTACGC-TATCTGAGATCATCGTGAAAGCTAGT---------------------------------------
cbrenneri	------------------------------------GTCGGTTTTCACCTTGATCTGAGAGCTAT---CTGAATGC-TATCTGAGATCATCGTGAAAGCTAGT---------------------------------------
ppacificus	------------------------TCACTCCCGGCATGCGGTTTTCACGTTGATCTGAGAGCTAA--TCGATATG--TTTCTGAGATCATAGTGAAAGCTACTTGCCGAAGTGA----------------------------
cremanei	TTGACTATTCTAAAACCACTGCCCTCGTGGCCAGCAGTCGGTTTTCACCTTGATCTGAGAGCTAA--ACATAATGCTTATCTGAGATCATCGTGAAAGCTAGTTGCAGGTACACAGGCTCTTTGACATTAATGAATAATCGA
	******** * ************ * * * * ********* ******** *
	----- --------------------------------------------------- --------------------------------------------------------- -----	T07D1.2.2 T07D1.2 T07D1.2
	----- --------------------------------------------------- --------------------------------------------------------- -----	T07D1.2.1 T07D1.2 T07D1.2
	----- --------------------------------------------------- --------------------------------------------------------- -----	T07D1.2.3 T07D1.2 T07D1.2
celegans	......(((..((((.(((((((..(((((((((.(((((((.((((((.. .......)))...))).))))))).)))))))))..)))))))..))))))).....	1.000 -43.60
cbriggsae	...(((((((((..((((((.((((((..........)))...))).))))))..)))))))))...	1.000 -23.70
cjaponica	...(((((((....((((((.(((((... ......)) ..))).))))))....)))))))...	1.000 -17.70
cbrenneri	...(((((((((..((((((.(((((... ......)) ..))).))))))..)))))))))...	1.000 -23.80
ppacificus	(((((..(((((...(((((((((..((((((.(((((... .......) )).)).))))))..)))))))))...))))).)))))	1.000 -36.00
cremanei	((((.(((((..........(((...((((((.((((..(((((((((..((((((.(((((... .......))...))).))))))..)))))))))..)))).))).))).)))..............))))).))))	0.918 -43.85

celegans	chromosome:X:2431067:2431174:1	Opposite_strand\|Intronic_coding\|T07D1.2.2\|T07D1.2 ## T07D1.2\|protein_coding\|T07D1.2\|T07D1.2 [Source:RefSeq_peptide;Acc:NP_508444] ## {SimpF: WRM069cB10 1 fosmid,WRM062aG12 1 fosmid,WRM0618aC04 1 fosmid,WRM0630cF03 1 fosmid,WRM0631bE05 1 fosmid,WRM0636aE07 1 fosmid} ## {MIR: cel-mir-81}
cbriggsae	chromosome:chrX:4426639:4426786:-1	Opposite_strand\|Intronic_coding\|NM_001087279 ## Same_strand\|Intronic_coding\|NM_001109816 ## {MIR: cbr-mir-81}
cjaponica	chromosome:chrUn:6896845:6896992:1	intergenic
cbrenneri	chromosome:chrUn:978926:979073:-1	Opposite_strand\|Intronic_coding\|NM_076043 ## {SimpF: trf}
ppacificus	chromosome:chrUn:128697181:128697266:-1	Same_strand\|Intronic_coding\|NM_022416 ## Opposite_strand\|Intronic_coding\|NM_001028815 ## {Repeats: GA-rich 1 89 -1 class=Low_complexity}
cremanei	chromosome:chrUn:34151995:34152134:-1	Opposite_strand\|Intronic_coding\|NM_076043

miR-Intess™

cel-mir-81

block8205 (miRBase cel-mir-81) [miRNAknown_cloningHIGH_randfoldOK]